Item no. |
PRS-76-928-25tests |
Manufacturer |
ProSci
|
Amount |
25 tests |
Category |
|
Type |
Antibody Monoclonal |
Format |
liquid |
Applications |
FC |
Specific against |
Human |
Host |
Mouse |
ECLASS 10.1 |
42030590 |
ECLASS 11.0 |
42030590 |
UNSPSC |
12352203 |
Alias |
ILRA, CD127, IL7RA, CDW127, IL-7R-alpha, IL7R |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Chemokines & Cytokines, Growth Factors |
Specificity |
The RDR5 monoclonal antibody specifically reacts with human CD127, a 60-90 kDA type I transmembrane glycoprotein also known as the IL-7 receptor alpha chain. |
Purification |
The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product. |
Clonality |
Monoclonal |
Clone |
RDR5 |
Isotype |
Mouse IgG1, kappa |
Conjugate |
PE-Cy5 |
Buffer |
Phosphate-buffered aqueous solution, ≤0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2. |
Concentration |
batch dependent |
Storage Conditions |
The product should be stored undiluted at 4˚ C and should be protected from prolonged exposure to light. Do not freeze. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
IL7R |
Ncbi Gene Id # |
3575 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
IL7R |
Ncbi Official Full Name |
interleukin 7 receptor |
Ncbi Organism |
Homo sapiens |
Swissprot # |
P16871 |
Background |
The RDR5 monoclonal antibody specifically reacts with human CD127, a 60-90 kDA type I transmembrane glycoprotein also known as the IL-7 receptor alpha chain. CD127 binds with CD132 (gamma c) to forms the IL-7 receptor that is expressed on mature T cells and immature B cells. Since CD127 expression is downregulated in Treg cells, it can be used as a marker for differentiating them from common T cells. The binding of the RDR5 antibody can be blocked by pre-incubating the cells with human IL-7. |
Background References 1 |
Sallusto, F., Lenig, D., Frster, R., Lipp, M., Lanzavecchia, A. (1999). Two subsets of memory T lymphocytes with distinct homing potentials and effector functions.Nature, 401(6754), 708-712. |
Background References 2 |
Yoshida, R., Nagira, M., Imai, T., Baba, M., Takagi, S., Tabira, Y., ... Yoshie, O. (1998). EBI1-ligand chemokine (ELC) attracts a broad spectrum of lymphocytes: activated T cells strongly up-regulate CCR7 and efficiently migrate toward ELC.International immunology, 10(7), 901-910. |
Background References 3 |
Lee, N., Llano, M., Carretero, M., Ishitani, A., Navarro, F., Lpez-Botet, M., Geraghty, D. E. (1998). HLA-E is a major ligand for the natural killer inhibitory receptor CD94/NKG2A.Proceedings of the National Academy of Sciences, 95(9), 5199-5204. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.