Item no. |
PRS-76-605-0.025mg |
Manufacturer |
ProSci
|
Amount |
0.025 mg |
Category |
|
Type |
Antibody Monoclonal |
Format |
liquid |
Applications |
FC |
Specific against |
Mouse |
Host |
Rat |
ECLASS 10.1 |
42030590 |
ECLASS 11.0 |
42030590 |
UNSPSC |
12352203 |
Alias |
B7, B70, MB7, B7-2, B7.2, CLS1, Ly58, ETC-1, Ly-58, MB7-2, Cd28l2, TS/A-2, Cd86 |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Innate Immunity |
Specificity |
The GL-1 monoclonal antibody reacts specifically with the mouse B7-2 (also known as CD86), a costimulatory molecule expressed by B and T lymphocytes, macrophages (thioglycollate-induced from peritoneum), astrocytes and dendritic cells. |
Purification |
The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product. |
Clonality |
Monoclonal |
Clone |
GL-1 (GL1) |
Isotype |
Rat IgG2a, kappa |
Conjugate |
APC |
Buffer |
Phosphate-buffered aqueous solution, ≤0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2. |
Concentration |
batch dependent |
Storage Conditions |
The product should be stored undiluted at 4˚ C and should be protected from prolonged exposure to light. Do not freeze. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
Cd86 |
Ncbi Gene Id # |
12524 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
Cd86 |
Ncbi Official Full Name |
CD86 antigen |
Ncbi Organism |
Mus musculus |
Swissprot # |
P42082 |
Background |
The GL-1 monoclonal antibody reacts specifically with the mouse B7-2 (also known as CD86), a costimulatory molecule expressed by B and T lymphocytes, macrophages (thioglycollate-induced from peritoneum), astrocytes and dendritic cells. The memory CD4+ T lymphocytes express CD86 (as mRNA and protein). CD86, and the B7-1 (CD 80) molecule, are ligands for CD152 and CD28, and influence the costimulatory interactions between lymphocytes B and T. B7-2 is also involved in the mouse natural killer cell-mediated cytotoxicity. The GL-1 antibody blocks the mixed lymphocyte reaction (MLR) and inhibits the T-cells stimulation by antigen-presenting cells. Mixtures of anti-B7-1 antibody and GL-1 were reported to inhibit the interaction between CD125 and its ligand with the in vivo priming of cytotoxic T lymphocytes. |
Background References 1 |
Maeda, K., Sato, T., Azuma, M., Yagita, H., Okumura, K. (1997). Characterization of rat CD80 and CD86 by molecular cloning and mAb.International immunology, 9(7), 993-1000. |
Background References 2 |
Damoiseaux, J. G., Yagita, H., Okumura, K., van Breda Vriesman, P. J. (1998). Costimulatory molecules CD80 and CD86 in the rat |
Background References 3 |
tissue distribution and expression by antigen-presenting cells.Journal of leukocyte biology, 64(6), 803-809. |
Background References 4 |
BLUESTONE, J. (1995). New perspectives of CD28-B7-mediated T cell costimulation.Immunity, 2, 555-559. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.