Item no. |
PRS-7581-0.02mg |
Manufacturer |
ProSci
|
Amount |
0.02 mg |
Category |
|
Type |
Antibody Polyclonal |
Format |
Liquid |
Applications |
WB, IF, ELISA, IHC-P |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
MAP1LC3B Antibody: LC3B, ATG8F, MAP1LC3B-a, MAP1A/1BLC3, MAP1ALC3, Autophagy-related protein LC3 B |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Autophagy |
Homology |
Predicted species reactivity based on immunogen sequence: Bovine: (100%) |
Immunogen |
MAP1LC3B antibody was raised against an 18 amino acid peptide near the amino terminus of human MAP1LC3B.
The immunogen is located within the first 50 amino acids of MAP1LC3B. |
Applications |
MAP1LC3B antibody can be used for detection of MAP1LC3B by Western blot at 1 - 2 μ g/ml. Antibody can also be used for Immunohistochemistry starting at 5 μ g/mL. For immunofluorescence start at 20 μ g/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. |
Specificity |
MAP1LC3B antibody is human, mouse and rat reactive. Multiple isoforms MAP1LC3B are known to exist. MAP1LC3B antibody is predicted to not cross-react with MAP1LC3A or MAP1LC3C |
Positive Control 1 |
Cat. No. 1303 - Human Brain Tissue Lysate |
Positive Control 2 |
Cat. No. 10-301 - Human Brain Tissue Slide |
Predicted Molecular Weight |
Predicted: 14 kDa
Observed: 14 kDa |
Purification |
MAP1LC3B antibody is affinity chromatography purified via peptide column. |
Clonality |
Polyclonal |
Isotype |
IgG |
Conjugate |
Unconjugated |
Buffer |
MAP1LC3B antibody is supplied in PBS containing 0.02% sodium azide. |
Concentration |
1 mg/mL |
Storage Conditions |
MAP1LC3B antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. |
Related Products |
Cat. No. 7581P – MAP1LC3B Peptide |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
MAP1LC3B |
Accession # |
NP_073729 |
Protein Gi # |
12383056 |
Ncbi Gene Id # |
81631 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
MAP1LC3B |
Ncbi Official Full Name |
microtubule-associated protein 1 light chain 3 beta |
Ncbi Organism |
homo sapiens |
Swissprot # |
Q9GZQ8 |
Background |
Microtubule-associated proteins (MAPs) regulate microtubule stability and play critical roles in neuronal development and plasticity (1). MAP1LC3B belongs to the MAP1 LC3 family and it includes 3 different light chains, LC1, LC2 and LC3 (2). MAP1LC3B is involved in formation of autophagosomal vacuoles (autophagosomes) (3). It is most abundant in heart, brain, skeletal muscle and testis. MAP1LC3B is essential for autophagy and associated to the autophagosome membranes after processing (4). |
Background References 1 |
Mandelkow E and Mandelkow EM. Microtubules and microtubule-associated proteins. Curr. Opin. Cell Biol. 1995; 7:72-81. |
Background References 2 |
Fink JK, Jones SM, Esposito C, et al. Human microtubule-associated protein 1A (MAP1A) gene: genomic organization, cDNA sequence, and developmental and tissue-specific expression. Genomics 1996; 35:577-85. |
Background References 3 |
Colecchia D, Strambi A, Sanzone S, et al. MAPK15/ERK8 stimulates autophagy by interacting with LC3 and GABARAP proteins. Autophagy 2012; 8:1724-40. |
Background References 4 |
Kabeya Y, Mizushima N, Ueno T, et al. LC3, a mammalian homolog of yeast Apg8p, is localized in autophagosome membrane after processing. EMBO J. 2000; 19:5720-8. |
1st Image Caption |
Western blot analysis of MAP1LC3B in human brain tissue lysate with MAP1LC3B antibody at 1 μ g/ml. |
2nd Image Caption |
Immunohistochemistry of MAP1LC3B in human brain tissue with MAP1LC3B antibody at 5 μ g/mL. |
3rd Image Caption |
Immunofluorescence of MAP1LC3B in human brain tissue with MAP1LC3B antibody at 20 μ g/mL. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.