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IL-36A Antibody

IL-36A Antibody

Item no.	PRS-7505-0.02mg
Manufacturer	ProSci
Amount	0.02 mg
Category	Neuroscience Aging & Neurological Disorders Antibodies Immunohistochemistry Western Blot ELISA Immunofluorescence Primary Antibodies
Type	Antibody Polyclonal
Format	Liquid
Applications	WB, IF, ELISA, IHC-P
Specific against	Human
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	IL-36A Antibody: FIL1, FIL1E, IL1F6, IL-1F6, IL1(EPSILON), FIL1(EPSILON), IL1E, Interleukin-36 alpha, FIL1 epsilon, IL-1 epsilon
Available
Shipping	blue ice or RT
By Research Area	Immunology
Immunogen	IL-36A antibody was raised against an 11 amino acid peptide near the carboxy terminus of human IL-36A. The immunogen is located within the last 50 amino acids of IL-36A.
Applications	IL-36A antibody can be used for detection of IL-36A by Western blot at 1 - 2 μ g/ml. Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity	IL-36A antibody is human specific. IL-36A antibody will not cross-react with IL-36B or IL-36G.
Positive Control 1	Cat. No. 1219 - MCF7 Cell Lysate
Positive Control 2	Cat. No. 10-301 - Human Brain Tissue Slide
Predicted Molecular Weight	Predicted: 17 kDa Observed: 16 kDa
Purification	IL-36A antibody is affinity chromatography purified via peptide column.
Clonality	Polyclonal
Isotype	IgG
Conjugate	Unconjugated
Buffer	IL-36A antibody is supplied in PBS containing 0.02% sodium azide.
Concentration	1 mg/mL
Storage Conditions	IL-36A antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year.
Related Products	Cat. No. 7505P – IL-36A Peptide
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	IL36A
Accession #	NP_055255
Protein Gi #	7657092
Ncbi Gene Id #	27179
User Note	Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol	IL36A
Ncbi Official Full Name	interleukin 36, alpha
Ncbi Organism	homo sapiens
Swissprot #	Q9UHA7
Background	IL-36A is is a member of the interleukin 1 cytokine family whose gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2 (1). IL-36A is thought to activate the NF-kappaB pathway through IL-1 receptor family members IL-1RL2 and IL-1RAcP (2). Like the related proteins IL-36B and IL-36G, IL-36A requires post-translational processing for full agonist activity, but the cleavage mechanism is currently unknown (3). The IL-36 cytokines have been suggested to amplify Th1 responses by enhancing proliferation and Th1 polarization of naive CD4+ T cells (4).
Background References 1	Smith DE, Renshaw BR, Ketchem RR, et al. Four new members expand the interleukin-1 superfamily. J. Biol. Chem. 2000; 275:1169-75.
Background References 2	Towne JE, Garka KE, Renshaw BR, et al. Interleukin (IL)-1F6, IL-1F8, and IL-1F9 signal through IL-1Rrp2 and IL-1RAcP to activate the pathway leading to NF-kappaB and MAPKs. J. Biol. Chem. 2004; 279:13677-88.
Background References 3	Towne JE, Renshaw BR, Douangpanya J, et al. Interleukin-36 (IL-36) ligands require processing for full agonist agonist (IL-36a, IL-36b, and IL-36g) or antagonist (IL-36Ra) activity. J. Biol. Chem. 2011; 286:42594-602.
Background References 4	Vigne S, Palmer G, Martin P, et al. IL-36 signaling amplifies Th1 responses by enhancing proliferation and Th1 polarization of naive CD4+ T cells. Blood 2012; 120:3478-87.
1st Image Caption	Western blot analysis of IL-36A in MCF7 cell lysate with IL-36A antibody at 1 μ g/ml in (A) the absence and (B) the presence of blocking peptide.
2nd Image Caption	Immunohistochemistry of IL-36A in human brain tissue with IL-36A antibody at 5 μ g/mL.
3rd Image Caption	Immunofluorescence of IL-36A in human brain tissue with IL-36A antibody at 20 μ g/mL.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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