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NELF Antibody

NELF Antibody

Item no.	PRS-7225-0.02mg
Manufacturer	ProSci
Amount	0.02 mg
Category	Oncology Antibodies Immunohistochemistry Western Blot ELISA Immunofluorescence Primary Antibodies By Organ Testicular & Prostate Cancer
Type	Antibody Polyclonal
Format	Liquid
Applications	WB, IF, ELISA, IHC-P
Specific against	Human, Mouse, Rat
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	NELF Antibody: HH9, NELF, NMDA receptor synaptonuclear signaling and neuronal migration factor, Nasal embryonic luteinizing hormone-releasing hormone factor, Nasal embryonic LHRH factor
Available
Shipping	blue ice or RT
By Research Area	Stem Cell
Immunogen	Rabbit polyclonal NELF antibody was raised against a 17 amino acid peptide near the center of human NELF. The immunogen is located within amino acids 230 - 280 of NELF.
Applications	NELF antibody can be used for detection of NELF by Western blot at 1 - 2 μ g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ g/mL. For immunofluorescence start at 20 μ g/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse and rat samples and Immunofluorescence in mouse and rat samples. All other applications and species not yet tested.
Positive Control 1	Cat. No. 1201 - HeLa Cell Lysate
Predicted Molecular Weight	Predicted: 58 kDa
Purification	NELF Antibody is affinity chromatography purified via peptide column.
Clonality	Polyclonal
Isotype	IgG
Conjugate	Unconjugated
Buffer	NELF Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration	1 mg/mL
Storage Conditions	NELF antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year.
Related Products	Cat. No. 7225P – NELF Peptide
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	NELF
Accession #	NP_056352
Protein Gi #	46397396
Ncbi Gene Id #	26012
User Note	Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol	NELF
Ncbi Official Full Name	nasal embryonic LHRH factor
Ncbi Organism	homo sapiens
Swissprot #	Q6X4W1
Background	NELF Antibody: NELF (nasal embryonic luteinizing hormone-releasing hormone factor) is a 530 amino acid transcription factor involved in the migration of LHRH neurons, outgrowth of olfactory axons and suppression of transcription elongation. NELF is found in the peripheral and central nervous system during embryonic development, and is highly expressed in adult testis, kidney and brain. Known to couple NMDA receptor signaling to the nucleus, NELF knockdown impaired GnRH neuronal migration of NLT cells in vitro and the gene encoding NELF has been linked to the development of Idiopathic hypogonadotropic hypogonadism (IHH), a disorder resulting in impaired pubertal maturation and reproductive function.
Background References 1	Kramer PR and Wray S. Novel gene expressed in nasal region influences outgrowth of olfactory axons and migration of luteinizing hormone-releasing hormone (LHRH) neurons. Genes Dev. 2000; 14:1824-34.
Background References 2	Yung TM, Narita T, Komori T, et al. Cellular dynamics of the negative transcription elongation factor NELF. Exp. Cell Res. 2009; 315:1693-705.
Background References 3	Xu N, Bhagavath B, Kim HG, et al. NELF is a nuclear protein involved in hypothalamic GnRH neuronal migration. Mol. Cell Endocrinol. 2010; 319:47-55.
Background References 4	Miura K, Acierno JS and Seminara SB. Characterization of the human nasal embryonic LHRH factor gene, NELF, and a mutation screening among 65 patients with idiopathic hypogonadotropic hypogonadism (IHH). J. Hum. Genet. 2004; 49: 265-8.
1st Image Caption	Western blot analysis of NELF in Hela cell lysate with NELF antibody at (A) 1 and (B) 2 μ g/mL.
2nd Image Caption	Immunohistochemistry of NELF in rat brain tissue with NELF antibody at 2.5 μ g/ml.
3rd Image Caption	Immunofluorescence of NELF in rat brain tissue with NELF antibody at 20 μ g/ml.
4th Image Caption	Immunohistochemistry of NELF in mouse brain tissue with NELF Antibody at 5 μ g/mL.
5th Image Caption	Immunofluorescence of NELF in mouse brain tissue with NELF Antibody at 20 μ g/mL. Green: NELF antibody (7225) Red: Phylloidin staining Blue: DAPI staining

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 0.02 mg

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Delivery expected until 9/6/2024

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