Item no. |
PRS-7093-0.02mg |
Manufacturer |
ProSci
|
Amount |
0.02 mg |
Category |
|
Type |
Antibody Polyclonal |
Format |
Liquid |
Applications |
WB, IF, ELISA |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
GDF1 Antibody: RAI, DORV, DTGA3GDF-1 |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Stem Cell |
Immunogen |
Rabbit polyclonal GDF1 antibody was raised against a 16 amino acid peptide near the amino terminus of human GDF1.
The immunogen is located within amino acids 30 - 80 of GDF1. |
Applications |
GDF1 antibody can be used for detection of GDF1 by Western blot at 1 - 2 μ g/mL. For immunofluorescence start at 20 μ g/mL.
Antibody validated: Western Blot in rat samples and Immunofluorescence in human samples. All other applications and species not yet tested. |
Specificity |
GDF1 antibody is predicted to not cross-react with any other members of the growth differentiation factor family. |
Positive Control 1 |
Cat. No. 1462 - Rat Lung Tissue Lysate |
Positive Control 2 |
Cat. No. 10-101 - Human Lung Tissue Slide |
Predicted Molecular Weight |
41 kDa |
Purification |
GDF1 Antibody is affinity chromatography purified via peptide column. |
Clonality |
Polyclonal |
Isotype |
IgG |
Conjugate |
Unconjugated |
Buffer |
GDF1 Antibody is supplied in PBS containing 0.02% sodium azide. |
Concentration |
1 mg/mL |
Storage Conditions |
GDF1 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Related Products |
Cat. No. 7093P – GDF1 Peptide |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
GDF1 |
Accession # |
NP_001483 |
Protein Gi # |
110349792 |
Ncbi Gene Id # |
2657 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
GDF1 |
Ncbi Official Full Name |
growth differentiation factor 1 |
Ncbi Organism |
homo sapiens |
Swissprot # |
P27539 |
Background |
GDF1 Antibody: Growth differentiation factors (GDFs) are members of the transforming growth factor (TGF) superfamily that is involved in embryonic development and adult tissue homeostasis. GDF1 was initially identified as a temporally expressed gene in the mouse central nervous system during embryonic development, with only one isoform detected in adult tissues. GDF1 is required for left-right patterning during development and directly interacts with Nodal, another member of the TGF-beta superfamily. It has been suggested that GDF1 regulates the activity and signaling range of Nodal through direct interaction. |
Background References 1 |
Massague J. 1990. The transforming growth factor-beta family. Ann. Rev. Cell Biol. 6:597-641. |
Background References 2 |
Lee SJ. Expression of growth/differentiation factor 1 in the nervous system: conservation of a bicistronic structure. Proc. Natl. Acad. Sci. USA 1991; 88:4250-4. |
Background References 3 |
Rankin CT, Bunton T, Lawler AM, et al. Regulation of left-right patterning in mice by growth/differentiation factor-1. Nat. Genet. 2000; 24:262-5. |
Background References 4 |
Tanaka C, Sakuma R, Nakamura T, et al. Long-range action of Nodal requires interaction with GDF1. Genes Dev. 2007; 21:3272-82. |
1st Image Caption |
Western blot analysis of GDF1 in rat lung tissue lysate with GDF1 antibody at (A) 1 and (B) 2 μ g/mL. |
3rd Image Caption |
Immunofluorescence of GDF1 in human lung tissue with GDF1 antibody at 20 μ g/mL. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.