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DLK1 Antibody

DLK1 Antibody

Item no.	PRS-7017-0.02mg
Manufacturer	ProSci
Amount	0.02 mg
Category	Metabolism Glucose Homeostasis Cholesterol & Lipid Metabolism Antibodies Western Blot ELISA Immunofluorescence Immunocytochemistry Primary Antibodies
Type	Antibody Polyclonal
Format	Liquid
Applications	WB, IF, ICC, ELISA
Specific against	Human, Mouse, Rat
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	DLK1 Antibody: DLK, Protein delta homolog 1, pG2, DLK-1
Available
Shipping	blue ice or RT
By Research Area	Stem Cell
Immunogen	DLK1 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human DLK1. The immunogen is located within amino acids 260 - 310 of DLK1.
Applications	DLK1 antibody can be used for detection of DLK1 by Western blot at 1 - 2 μ g/mL. Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity	At least four isoforms of DLK1 are known to exist.
Positive Control 1	Cat. No. 1211 - HepG2 Cell Lysate
Positive Control 2	Cat. No. 17-011 - Hep G2 Cell Slide
Predicted Molecular Weight	42 kDa
Purification	DLK1 Antibody is affinity chromatography purified via peptide column.
Clonality	Polyclonal
Isotype	IgG
Conjugate	Unconjugated
Buffer	DLK1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration	1 mg/mL
Storage Conditions	DLK1 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Related Products	Cat. No. 7017P – DLK1 Peptide
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	DLK1
Accession #	NP_003827
Protein Gi #	74136023
Ncbi Gene Id #	8788
User Note	Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol	DLK1
Ncbi Official Full Name	delta-like 1 homolog (Drosophila)
Ncbi Organism	homo sapiens
Swissprot #	P80370
Background	DLK1 Antibody: The Delta-like 1 homolog (DLK1) is a transmembrane protein containing six epidermal growth factor repeats. It is involved in the differentiation of several cell types, including adipocytes and is also thought to be a tumor suppressor. The DLK1 gene is one of several imprinted genes located in a region on chromosome 14q32; certain mutations in this imprinted region can cause phenotypes similar to maternal and paternal uniparental disomy of chromosome 14 (UPD14). DLK1 is expressed from the paternal allele; a polymorphism within this gene has been associated with child and adolescent obesity.
Background References 1	Smas CM and Sul HS. Pref-1, a protein containing EGF-like repeats, inhibits adipocyte differentiation. Cell 1993; 73:725-34.
Background References 2	Sul HS. Mini-review: Pref-1: role in adipogenesis and mesenchymal cell fate. Mol. Endocrinol. 2009; 23:1717-25.
Background References 3	Kawakami T, Chano T, Minami K, et al. Imprinted DLK1 is a putative tumor suppressor gene and inactivated by epimutation at the region upstream of GTL2 in human renal cell carcinoma. Hum. Mol. Genet. 2006; 15:821-30.
Background References 4	Wermter AK, Scherag A, Meyre D, et al. Preferential reciprocal transfer of paternal/maternal DLK1 alleles to obese children: first evidence of polar overdomainance in human. Eur. J. Hum. Genet. 2008; 16:1126-34.
1st Image Caption	Western blot analysis of HepG2 in 293 cell lysate with DLK1 antibody at (A) 1 and (B) 2 μ g/mL.
2nd Image Caption	Immunocytochemistry of DLK1 in HepG2 cells with DLK1 antibody at 2.5 μ g/ml.
3rd Image Caption	Immunofluorescence of DLK1 in HepG2 cells with DLK1 antibody at 20 μ g/ml.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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