Item no. |
PRS-6955-0.02mg |
Manufacturer |
ProSci
|
Amount |
0.02 mg |
Category |
|
Type |
Antibody Polyclonal |
Format |
Liquid |
Applications |
WB, IF, ELISA |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
TCTEX1D3 Antibody: TCTEX1D3, TCTEX1D3, Tctex1 domain-containing protein 3, T-complex testis-specific protein 3, Tcte-3 |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Homeostasis |
Immunogen |
TCTEX1D3 antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human TCTEX1D3.
The immunogen is located within the first 50 amino acids of TCTEX1D3. |
Applications |
TCTEX1D3 antibody can be used for detection of TCTEX1D3 by Western blot at 1 μ g/mL. For immunofluorescence start at 20 μ g/mL.
Antibody validated: Western Blot in mouse samples and Immunofluorescence in human samples. All other applications and species not yet tested. |
Specificity |
TCTEX1D3 is predicted to not cross-react with other members of the TCTEX-type family |
Positive Control 1 |
Cat. No. 1287 - EL4 Cell Lysate |
Positive Control 2 |
Cat. No. 11-701 - Human Testis Tissue Slide |
Predicted Molecular Weight |
22 kDa |
Purification |
TCTEX1D3 Antibody is affinity chromatography purified via peptide column. |
Clonality |
Polyclonal |
Isotype |
IgG |
Conjugate |
Unconjugated |
Buffer |
TCTEX1D3 Antibody is supplied in PBS containing 0.02% sodium azide. |
Concentration |
1 mg/mL |
Storage Conditions |
TCTEX1D3 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Related Products |
Cat. No. 6955P – TCTEX1D3 Peptide |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
TCTE3 |
Accession # |
NP_777570 |
Protein Gi # |
28372535 |
Ncbi Gene Id # |
6991 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
TCTE3 |
Ncbi Official Full Name |
t-complex-associated-testis-expressed 3 |
Ncbi Organism |
homo sapiens |
Swissprot # |
Q8IZS6 |
Background |
TCTEX1D3 Antibody: Dyneins are multi-subunit, high molecular weight ATPases that interact with microtubules to generate force by converting the chemical energy of ATP into the mechanical energy of movement. Cytoplasmic or axonemal Dynein is an approximately 12 subunit complex of two heavy chains, two intermediate chains to anchor Dynein to its cargo, four smaller intermediate chains and several light chains. It performs functions necessary for cell survival such as organelle transport and centrosome assembly. TCTEX1 is a cytoplasmic dynein light chain found in a complex with Na+ CP type X (SCN10A). TCTEX1D3, also called TCTE3, has been identified as a gene responsible for congenital diaphragmatic hernia (CDH). |
Background References 1 |
He Y, Francis F, Myers KA, et al. Role of cytoplasmic dynein in the axonal transport of microtubules and neurofilaments. J. Cell Biol. 2005; 168:697-703. |
Background References 2 |
Li J, Lee WL, and Cooper JA. NudEL targets dynein to microtubule ends through LIS1. Nat. Cell Biol. 2005; 7:686-90. |
Background References 3 |
Watanabe TK, Fujiwara T, Shimizu F, et al. Cloning, expression, and mapping of TCTEL1, a putative human homologue of murine Tcte1 to 6q. Cytogenet. Cell Genet. 1996; 73:153-6. |
Background References 4 |
Chuang JZ, Yeh TY, Bollati F, et al. The dynein light chain Tctex-1 has a dynein-independent role in actin remodeling during neurite outgrowth. Dev. Cell 2005; 9:75-86. |
1st Image Caption |
Western blot analysis of TCTEX1D3 in EL4 cell lysate with TCTEX1D3 antibody at 1 μ g/mL in (A) the absence and (B) the presence of blocking peptide. |
3rd Image Caption |
Immunofluorescence of TCTEX1D3 in human testis tissue with TCTEX1D3 antibody at 20 μ g/mL. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.