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SDHAF1 Antibody

SDHAF1 Antibody

Item no.	PRS-6879-0.1mg
Manufacturer	ProSci
Amount	0.1 mg
Category	Antibodies Western Blot ELISA Immunofluorescence Immunocytochemistry Primary Antibodies
Type	Antibody Polyclonal
Format	Liquid
Applications	WB, IF, ICC, ELISA
Specific against	Human, Mouse
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	SDHAF1 Antibody: LYRM8, LYRM8, Succinate dehydrogenase assembly factor 1, mitochondrial, LYR motif-containing protein 8, SDH assembly factor 1
Available
Shipping	blue ice or RT
By Research Area	Homeostasis
Immunogen	SDHAF1 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human SDHAF1. The immunogen is located within the last 50 amino acids of SDHAF1.
Applications	SDHAF1 antibody can be used for detection of SDHAF1 by Western blot at 1 - 2 μ g/mL. Antibody can also be used for immunocytochemistry starting at 5 μ g/mL. For immunofluorescence start at 5 μ g/mL. Antibody validated: Western Blot in mouse samples; Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Specificity	SDHAF1 antibody is predicted to not cross-react with other SDHAF protein family members.
Positive Control 1	Cat. No. 1282 - 3T3 (NIH) Cell Lysate
Positive Control 2	Cat. No. 17-201 - 3T3/BALB Cell Slide
Purification	SDHAF1 Antibody is affinity chromatography purified via peptide column.
Clonality	Polyclonal
Isotype	IgG
Conjugate	Unconjugated
Buffer	SDHAF1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration	1 mg/mL
Storage Conditions	SDHAF1 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Related Products	Cat. No. 6879P – SDHAF1 Peptide
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	SDHAF1
Accession #	NP_001036096
Protein Gi #	111038124
Ncbi Gene Id #	644096
User Note	Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol	SDHAF1
Ncbi Official Full Name	succinate dehydrogenase complex assembly factor 1
Ncbi Organism	Homo sapiens
Swissprot #	A6NFY7
Background	SDHAF1 Antibody: SDHAF1 (Succinate dehydrogenase complex assembly factor 1) is one of the subunits of the succinate dehydrogenase (SDH) complex (or complex II) of the mitochondrial respiratory chain. SDHAF1 resides in the mitochondria, and is essential for SDH assembly, but does not physically associate with the complex in vivo. Mutations in this gene are associated with SDH-defective infantile leukoencephalopathy (mitochondrial complex II deficiency). Unlike the related protein SDHAF2, SDHAF1 is not thought to be a tumor suppressor.
Background References 1	Ghezzi D, Goffrini P, Uziel G, et al. SDHAF1, encoding a LYR complex-II specific assembly factor, is mutated in SDH-defective infantile leukoencephalopathy. Nat. Genet. 2009; 41:654-6.
Background References 2	Hensen EE and Bayley JP. Recent advances in the genetics of SDH-related paraganglioma and pheochromocytoma. Fam. Cancer 2011; 10:355-63.
1st Image Caption	Western blot analysis of SDHAF1 in 3T3 cell lysate with SDHAF1 antibody at (A) 1 and (B) 2 μ g/mL.
2nd Image Caption	Immunocytochemistry of SDHAF1 in 3T3 cells with SDHAF1 antibody at 5 μ g/mL.
3rd Image Caption	Immunofluorescence of SDHAF1 in 3T3 cells with SDHAF1 antibody at 20 μ g/mL.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 0.1 mg

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Delivery expected until 9/6/2024

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