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NIBRIN Antibody

NIBRIN Antibody

Item no.	PRS-6839-0.1mg
Manufacturer	ProSci
Amount	0.1 mg
Category	Oncology Antibodies By Cell Type Blastoma Carcinoma Hematology Lymphoma Myeloma Melanoma Sarcoma Immunohistochemistry Western Blot ELISA Immunofluorescence Primary Antibodies By Organ Breast Cancer
Type	Antibody Polyclonal
Format	Liquid
Applications	WB, IF, ELISA, IHC-P
Specific against	Human, Mouse
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	NIBRIN Antibody: ATV, NBS, P95, NBS1, AT-V1, AT-V2, Nibrin, Cell cycle regulatory protein p95
Available
Shipping	blue ice or RT
By Research Area	Cancer, Cell Cycle
Homology	Predicted species reactivity based on immunogen sequence: Rat: (93%)
Immunogen	NIBRIN antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human NIBRIN. The immunogen is located within the last 50 amino acids of NIBRIN.
Applications	NIBRIN antibody can be used for detection of NIBRIN by Western blot at 1 μ g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μ g/mL. For immunofluorescence start at 2.5 μ g/mL. Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity	Two alternatively spliced transcript isoforms of NIBRIN are known to exist.
Positive Control 1	Cat. No. 1402 - Mouse Lung Tissue Lysate
Positive Control 2	Cat. No. 10-101 - Human Lung Tissue Slide
Purification	NIBRIN Antibody is affinity chromatography purified via peptide column.
Clonality	Polyclonal
Isotype	IgG
Conjugate	Unconjugated
Buffer	NIBRIN Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration	1 mg/mL
Storage Conditions	NIBRIN antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Related Products	Cat. No. 6839P – NIBRIN Peptide
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	NBN
Accession #	NP_002476
Protein Gi #	33356172
Ncbi Gene Id #	4683
User Note	Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol	NBN
Ncbi Official Full Name	nibrin
Ncbi Organism	Homo sapiens
Swissprot #	O60934
Background	NIBRIN Antibody: NIBRIN (NBN) is a member of the double-strand break repair complex MRE11/RAD50/NBN (MRN) which is involved in DNA double-strand break repair, DNA damage-induced checkpoint activation and plays a critical role in the maintenance of chromosome integrity. NIBRIN contains two modules found in cell cycle checkpoint proteins, a forkhead-associated domain adjacent to a breast cancer carboxy-terminal domain. Mutations in this gene are associated with Nijmegen breakage syndrome and maybe the cause of cancer predisposition and aplastic anemia.
Background References 1	Carney JP, Maser RS, Olivares H, et al. The hMre11/hRad50 protein complex and Nijmegen breakage syndrome: linkage of double-strand break repair to the cellular DNA damage response. Cell 1998; 93:477-86
Background References 2	Marcelain K, De La Torre C, Gonzalez P, et al. Roles of nibrin and AtM/ATR kinases on the G2 checkpoint under endogenous or radio-induced DNA damage. Biol. Res. 2005; 38:179-85.
Background References 3	Varon R, Vissinga C, Platzer M, et al. Nibrin, a novel DNA double-strand break repair protein, is mutated in Nijmegen breakage syndrome. Cell 1998; 93:467-76.
Background References 4	Heikkinen K, Karppinen SM, Soini Y. et al. Mutation screening of Mre11 complex genes: indication of RAD50 involvement in breast and ovarian cancer susceptibility. J. Med. Genet. 2003; 40:E131.
1st Image Caption	Western blot analysis of NIBRIN in mouse lung tissue lysate with NIBRIN antibody at 1 μ g/mL.
2nd Image Caption	Immunohistochemistry of NIBRIN (CT) in human lung tissue with NIBRIN (CT) antibody at 2.5 μ g/mL.
3rd Image Caption	Immunofluorescence of NIBRIN in human lung tissue with NIBRIN antibody at 20 μ g/mL.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 0.1 mg

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Delivery expected until 9/6/2024

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