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RILP Antibody

RILP Antibody

Item no.	PRS-6791-0.02mg
Manufacturer	ProSci
Amount	0.02 mg
Category	Antibodies Western Blot ELISA Immunofluorescence Immunocytochemistry Primary Antibodies
Type	Antibody Polyclonal
Format	Liquid
Applications	WB, IF, ICC, ELISA
Specific against	Human, Mouse
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	RILP Antibody: PP10141, Rab-interacting lysosomal protein
Available
Shipping	blue ice or RT
By Research Area	Homeostasis
Immunogen	RILP antibody was raised against an 18 amino acid synthetic peptide near the center of human RILP. The immunogen is located within amino acids 110 - 160 of RILP.
Applications	RILP antibody can be used for detection of RILP by Western blot at 1 μ g/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μ g/mL. For immunofluorescence start at 2.5 μ g/mL. Antibody validated: Western Blot in mouse samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1	Cat. No. 1288 - A20 Cell Lysate
Positive Control 2	Cat. No. 17-001 - HeLa Cell Slide
Purification	RILP Antibody is affinity chromatography purified via peptide column.
Clonality	Polyclonal
Isotype	IgG
Conjugate	Unconjugated
Buffer	RILP Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration	1 mg/mL
Storage Conditions	RILP antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Related Products	Cat. No. 6791P – RILP Peptide
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	RILP
Accession #	NP_113618
Protein Gi #	93204881
Ncbi Gene Id #	83547
User Note	Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol	RILP
Ncbi Official Full Name	Rab interacting lysosomal protein
Ncbi Organism	Homo sapiens
Swissprot #	Q96NA2
Background	RILP Antibody: The Rab interacting lysosomal protein (RILP) is lysosomal protein that interacts with RAB7, a small GTPase that controls transport to endocytic degradative compartments. It is thought that RILP is a downstream effector of RAB7 and both proteins act together in the regulation of late endocytic traffic. Overexpression of RILP caused enlarged lysosomes that are more centrally located in the cell suggesting that RILP may also be involved in the regulation of lysosomal morphology. Other evidence suggests that RILP may play a role in the biogenesis of multivesicular bodies.
Background References 1	Cantalupo G, Alifano P, Roberti V, et al. Rab-interacting lysosomal protein (RILP): the Rab7 effector required for transport to lysosomes. EMBO J. 2001; 20:683-93.
Background References 2	Wang T, Wong KK, and Hong W. A unique region of RILP distinguishes it from its related proteins in its regulation of lysosomal morphology and interaction with Rab7 and Rab34. Mol. Biol. Cell 2004; 15:815-26.
Background References 3	Progida C, Spinosa MR, De Luca A, et al. RILP interacts with the VPS22 component of the ESCRT-II complex. Biochem. Biophys. Res. Commun. 2006; 347:1074-9.
1st Image Caption	Western blot analysis of RILP in A20 cell lysate with RILP antibody at 1 μ g/mL.
2nd Image Caption	Immunocytochemistry of RILP in HeLa cells with RILP antibody at 2.5 μ g/mL.
3rd Image Caption	Immunofluorescence of RILP in HeLa cells with RILP antibody at 20 μ g/mL.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 0.02 mg

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Delivery expected until 10/4/2024

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