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Periphilin Antibody

Periphilin Antibody

Item no.	PRS-6687-0.1mg
Manufacturer	ProSci
Amount	0.1 mg
Category	Oncology Antibodies Immunohistochemistry Western Blot ELISA Immunofluorescence Primary Antibodies By Organ Gastric Cancer
Type	Antibody Polyclonal
Format	Liquid
Applications	WB, IF, ELISA, IHC-P
Specific against	Human, Mouse
Host	Rabbit
ECLASS 5.1	32160702
ECLASS 6.1	32160702
ECLASS 8.0	32160702
ECLASS 9.0	32160702
ECLASS 10.0.1	32160702
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	Periphilin Antibody: HSPC206, HSPC232, Periphilin-1, Gastric cancer antigen Ga50
Available
Shipping	blue ice or RT
By Research Area	Cancer, Cell Cycle
Immunogen	Periphilin antibody was raised against a 16 amino acid synthetic peptide near the center of human Periphilin. The immunogen is located within amino acids 160 - 210 of Periphilin.
Applications	Periphilin antibody can be used for detection of Periphilin by Western blot at 1 μ g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μ g/mL. For immunofluorescence start at 5 μ g/mL. Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1	Cat. No. 1411 - Mouse Colon Tissue Lysate
Purification	Periphilin Antibody is affinity chromatography purified via peptide column.
Clonality	Polyclonal
Isotype	IgG
Conjugate	Unconjugated
Buffer	Periphilin Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration	1 mg/mL
Storage Conditions	Periphilin antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Related Products	Cat. No. 6687P – Periphilin Peptide
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	PPHLN1
Accession #	NP_958848
Protein Gi #	48255929
Ncbi Gene Id #	51535
User Note	Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol	PPHLN1
Ncbi Official Full Name	periphilin 1
Ncbi Organism	Homo sapiens
Swissprot #	Q8NEY8
Background	Periphilin Antibody: Periphilin, known as PPHLN1 or gastric cancer antigen Ga50, plays a role in epithelial differentiation and contributes to epidermal integrity and barrier formation. It interacts with periplakin, a known precursor of the cornified cell envelope. Periphilin 1 is ubiquitously expressed and localizes to nucleus and cytoplasm. Existing as eight alternatively spliced isoforms, Periphilin is highly insoluble and contains a putative nuclear localization signal (NLS) within its N-terminal half, a prerequisite for the formation of insoluble complexes and a possible caspase recognition sequence and a potential nuclear export signal. Periphilin may play an important role in the nervous system.
Background References 1	Kazerounian S and Aho S. Characterization of periphilin, a widespread, highly insoluble nuclear protein and potential constituent of the keratinocyte cornified envelope. J. Biol. Chem. 2003; 278:36707-17.
Background References 2	Kurita M, Suzuki H, Masai H, et al. Overexpression of CR/periphilin downregulates Cdc7 expression and induces S-phase arrest. Biochem. Biophys. Res. Commun. 2004; 324:554-61
Background References 3	Soehn AS, Pham TT, Schaeferhoff K, et al. Periphilin is strongly expressed in the murine nervous system and is indispensable for murine development. Genesis 2009; 47:697-707
Background References 4	Soehn AS, Franck T, Biskup S, et al. Periphilin is a novel interactor of synphilin-1, a protein implicated in Parkinson's disease. Neurogenetics 2010; 11:203-15
1st Image Caption	Western blot analysis of Periphilin in mouse colon tissue lysate with Periphilin antibody at 1 μ g/mL in (A) the absence and (B) the presence of blocking peptide.
2nd Image Caption	Immunohistochemistry of Periphilin in human colon tissue with Periphilin antibody at 2.5 μ g/mL.
3rd Image Caption	Immunofluorescence of Periphilin in human colon tissue with Periphilin antibody at 5 μ g/mL.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 0.1 mg

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Delivery expected until 9/6/2024

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