« Back
Home /
TGM5 Antibody

TGM5 Antibody

Item no.	PRS-6537-0.02mg
Manufacturer	ProSci
Amount	0.02 mg
Category	Antibodies Immunohistochemistry Western Blot ELISA Immunofluorescence Primary Antibodies
Type	Antibody Polyclonal
Format	Liquid
Applications	WB, IF, ELISA, IHC-P
Specific against	Human, Mouse, Rat
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	TGM5 Antibody: TGX, TGM6, TGMX, TGASE5, TGASEX, Protein-glutamine gamma-glutamyltransferase 5, Transglutaminase X, TGX
Available
Shipping	blue ice or RT
By Research Area	Apoptosis
Immunogen	TGM5 antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human TGM5. The immunogen is located within the last 50 amino acids of TGM5.
Applications	TGM5 antibody can be used for detection of TGM5 by Western blot at 1 - 2 μ g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μ g/mL. For immunofluorescence start at 20 μ g/mL. Antibody validated: Western Blot in rat samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Specificity	TGM5 antibody is predicted to not cross-react with other TGase protein family members. Multiple isoforms of TGM5 are known to exist.
Positive Control 1	Cat. No. 1461 - Rat Heart Tissue Lysate
Purification	TGM5 Antibody is affinity chromatography purified via peptide column.
Clonality	Polyclonal
Isotype	IgG
Conjugate	Unconjugated
Buffer	TGM5 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration	1 mg/mL
Storage Conditions	TGM5 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Related Products	Cat. No. 6537P – TGM5 Peptide
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	TGM5
Accession #	NP_963925
Protein Gi #	94981556
Ncbi Gene Id #	9333
User Note	Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol	TGM5
Ncbi Official Full Name	transglutaminase 5
Ncbi Organism	Homo sapiens
Swissprot #	O43548
Background	TGM5 Antibody: Transglutaminases (TGM) are a family of structurally and functionally related Ca2+-dependent enzymes (TGases) that stabilize protein assemblies through the formation of gamma-glutamyl-epsilon lysine crosslinks. TGases influence numerous biological processes, including blood coagulation, cell differentiation, fertilization and apoptosis. TGM5 belongs to the transglutaminase superfamily and catalyzes the cross-linking of proteins between glutamine and lysine residues, often resulting in stabilization of protein assemblies. TGM5 is also expressed in the human hair follicle. Defects in TGM5 are associated with acral peeling skin syndrome.
Background References 1	Ueki S, et al. Dual functions of transglutaminase in novel cell adhesion. J. Cell Sci. 1996; 109:2727-35.
Background References 2	Candi E, Oddi S, Terrinoni A, et al. Transglutaminase 5 cross-links loricrin, involicrin, and small proline-rich proteins in vitro. J. Biol. Chem. 2001; 276:35014-23.
Background References 3	Thibaut S, Candi E, Pietroni V, et al. Transglutaminase 5 expression in human hair follicle. J. Invest. Dermatol. 2005; 125:581-5
Background References 4	Cassidy AJ, van Steensel MA, Steijlen PM, et al. A homozygous missense mutation in TGM5 abolishes epidermal transglutaminase 5 activity and causes acral peeling skin syndrome. Am. J. Hum. Genet. 2005; 77:909-17.
1st Image Caption	Western blot analysis of TGM5 in rat heart tissue lysate with TGM5 antibody at (A) 1 and (B) 2 μ g/mL.
2nd Image Caption	Immunohistochemistry of TGM5 in mouse heart tissue with TGM5 antibody at 2.5 μ g/mL.
3rd Image Caption	Immunofluorescence of TGM5 in mouse heart tissue with TGM5 antibody at 20 μ g/mL.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

Request product datasheet

Request safety datasheet

Simple Order Order Subscription

Amount: 0.02 mg

available

Delivery expected until 9/6/2024

Express Delivery: Save 21 days

Compare

Add to wishlist

Recommend by mail