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SEC62 Antibody

SEC62 Antibody

Item no.	PRS-6471-0.02mg
Manufacturer	ProSci
Amount	0.02 mg
Category	Antibodies Immunohistochemistry Western Blot ELISA Immunofluorescence Primary Antibodies
Type	Antibody Polyclonal
Format	Liquid
Applications	WB, IF, ELISA, IHC-P
Specific against	Human, Mouse, Rat
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	SEC62 Antibody: HTP1, TP-1, Dtrp1, TLOC1, Translocation protein SEC62, Translocation protein 1
Available
Shipping	blue ice or RT
By Research Area	Homeostasis
Homology	Predicted species reactivity based on immunogen sequence: Chicken: (100%)
Immunogen	SEC62 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human SEC62. The immunogen is located within amino acids 270 - 320 of SEC62.
Applications	SEC62 antibody can be used for detection of SEC62 by Western blot at 0.5 - 1 μ g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ g/mL. For immunofluorescence start at 20 μ g/mL. Antibody validated: Western Blot in rat samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Specificity	SEC62 antibody is predicted to not cross-react with other SEC proteins. Multiple isoforms of SEC62 are known to exist.
Positive Control 1	Cat. No. 1463 - Rat Brain Tissue Lysate
Purification	SEC62 Antibody is affinity chromatography purified via peptide column.
Clonality	Polyclonal
Isotype	IgG
Conjugate	Unconjugated
Buffer	SEC62 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration	1 mg/mL
Storage Conditions	SEC62 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Related Products	Cat. No. 6471P – SEC62 Peptide
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	SEC62
Accession #	NP_003253
Protein Gi #	4507525
Ncbi Gene Id #	7095
User Note	Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol	SEC62
Ncbi Official Full Name	SEC62 homolog (S. cerevisiae)
Ncbi Organism	Homo sapiens
Swissprot #	Q99442
Background	SEC62 Antibody: SEC62 is an integral membrane protein located in the rough endoplasmic reticulum (ER) and is part of the SEC61-SEC62-SEC63 complex that is the central component of the protein translocation apparatus of the ER membrane. It is speculated that SEC61-SEC62-SEC63 may perform post-translational protein translocation into the ER and might also perform the backward transport of ER proteins that are subject to the ubiquitin-proteasome-dependent degradation pathway. Silencing of this gene with RNAi dramatically reduced the migration and invasive potential of numerous tumor cell lines, suggesting that it may be an attractive target for therapy of various tumors.
Background References 1	Deshaies RJ and Scheckman R. SEC62 encodes a putative membrane protein required for protein translocation in the yeast endoplasmic reticulum. J. Cell Biol. 1989; 109:2653-64.
Background References 2	Meyer HA, Grau H, Kraft R, et al. Mammalian Sec61 is associated with Sec62 and Sec63. J. Biol. Chem. 2000; 275:14550-7
Background References 3	Greiner M, Kreutzer B, Jung V, et al. Silencing of the SEC62 gene inhibits migratory and invasive potential of various tumor cells. Int. J. Cancer 2011; 128:2284-95.
1st Image Caption	Western blot analysis of SEC62 in rat brain tissue lysate with SEC62 antibody at (A) 0.5 and (B) 1 μ g/mL.
2nd Image Caption	Immunohistochemistry of SEC62 in mouse brain tissue with SEC62 antibody at 5 μ g/mL.
3rd Image Caption	Immunofluorescence of SEC62 in mouse brain tissue with SEC62 antibody at 20 μ g/mL.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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