PIM2 Antibody

Cancer, Signal Transduction

For WB starting dilution is: 1:1000

For IP starting dilution is: 1:50~100

For IHC-P starting dilution is: 1:50~100

This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

Rabbit Ig

Supplied in PBS with 0.09% (W/V) sodium azide.

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

None

Q9P1W9

11040

PIM2

Homo sapiens

Pim proteins (Pim-1, Pim-2 and Pim-3) are oncogene-encoded serine/threonine kinases. Pim-2 is highly homologous to Pim-1 with similar oncogenic functions. Pim-2 overexpression promotes resistance to a host of apoptotic stimuli; its expression is negatively regulated by growth factor depletion. Increased levels of Pim-2 has also been observed in certain cancers.

Baytel, D., et al., Biochim. Biophys. Acta 1442 (2-3), 274-285 (1998).

Western blot analysis of anti-PIM2 Antibody in Hela cell line lysates (35ug/lane)

PIM proteins were immunoprecipitated from MV4; 11 cells and the agarose-protein A-immunoprecipitate complex was tested for its ability to phosphorylate BAD in vitro in the presence or absence of K00135. Phosphorylation of BAD (both on Ser112 and Ser136, detected by WB with phospho-specific antibodies) was abrogated on addition of the compound. Asterisks, strong bands corresponding to the heavy chain of the anti-PIM2 rabbit antibody recognized by the antirabbit immunoglobulin G secondary antibody. Beads alone (without anti-PIM antibodies) were incubated with the MV4; 11 extract and used for the same in vitro phosphorylation reaction as a negative control.