Manufacturer |
ProSci
|
Category |
|
Type |
Antibody Polyclonal |
Specific against |
Human, Mouse |
Format |
Liquid |
Applications |
WB, IHC-P |
Amount |
400 ul |
Host |
Rabbit |
Item no. |
PRS-63-044-400ul |
eClass 6.1 |
32160702 |
eClass 9.0 |
32160702 |
Available |
|
Alias |
Ephrin type-A receptor 5, Brain-specific kinase, EPH homology kinase 1, EHK-1, EPH-like kinase 7, EK7, hEK7, EPHA5, BSK, EHK1, HEK7, TYRO4 |
Shipping |
blue ice or RT |
By Research Area |
Neuroscience, Signal Transduction |
Immunogen |
This EphA5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 17-42 amino acids from the N-terminal region of human EphA5. |
Applications |
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50 |
Predicted Molecular Weight |
115 kDa |
Purification |
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis |
Clonality |
Polyclonal |
Isotype |
Rabbit Ig |
Conjugate |
Unconjugated |
Buffer |
Supplied in PBS with 0.09% (W/V) sodium azide. |
Concentration |
batch dependent |
Storage Conditions |
Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
EPHA5 |
Accession # |
P54756 |
Protein Gi # |
259016353 |
Ncbi Gene Id # |
2044 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
EPHA5 |
Ncbi Official Full Name |
Ephrin type-A receptor 5 |
Ncbi Organism |
Homo sapiens |
Swissprot # |
P54756 |
Background |
Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The tyrosine kinase (TK) group is mainly involved in the regulation of cell-cell interactions such as differentiation, adhesion, motility and death. There are currently about 90 TK genes sequenced, 58 are of receptor protein TK (e.g. EGFR, EPH, FGFR, PDGFR, TRK, and VEGFR families), and 32 of cytosolic TK (e.g. ABL, FAK, JAK, and SRC families). |
Background References 1 |
Pejovic, T. et al., Transl Oncol. Dec 01; 2(4): 341-9(2009). |
Background References 2 |
Fox, G.M., et al., Oncogene 10(5):897-905 (1995). |
1st Image Caption |
Western blot analysis of anti-EphA5 N-term Pab in mouse brain tissue |
2nd Image Caption |
Formalin-fixed and paraffin-embedded human brain tissue reacted with EphA5 antibody (N-term ), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.