Item no. |
PRS-62-933-400ul |
Manufacturer |
ProSci
|
Amount |
400 ul |
Category |
|
Type |
Antibody Polyclonal |
Format |
Liquid |
Applications |
WB, FC, IHC-P |
Specific against |
Human |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
Vitronectin, VN, S-protein, Serum-spreading factor, V75, Vitronectin V65 subunit, Vitronectin V10 subunit, Somatomedin-B, VTN |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Immunology |
Immunogen |
This VTN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 352-379 amino acids from the C-terminal region of human VTN. |
Applications |
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50 |
Predicted Molecular Weight |
54 kDa |
Purification |
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis |
Clonality |
Polyclonal |
Isotype |
Rabbit Ig |
Conjugate |
Unconjugated |
Buffer |
Supplied in PBS with 0.09% (W/V) sodium azide. |
Concentration |
batch dependent |
Storage Conditions |
Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
VTN |
Accession # |
P04004 |
Protein Gi # |
139653 |
Ncbi Gene Id # |
7448 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
VTN |
Ncbi Official Full Name |
Vitronectin |
Ncbi Organism |
Homo sapiens |
Swissprot # |
P04004 |
Background |
VTN is a member of the pexin family. This protein is found in serum and tissues and promotes cell adhesion and spreading, inhibits the membrane-damaging effect of the terminal cytolytic complement pathway, and binds to several serpin serine protease inhibitors. The protein is a secreted protein and exists in either a single chain form or a clipped, two chain form held together by a disulfide bond. |
Background References 1 |
Jenne D.E., Stanley K.K.EMBO J. 4:3153-3157(1985) |
Background References 2 |
Sigurdardottir O., Wiman B.Biochim. Acta 1208:104-110(1994) |
Background References 3 |
Seiffert D., Loskutoff D.J.J. Biol. Chem. 266:2824-2830(1991) |
1st Image Caption |
Western blot analysis of VTN Antibody in NCI-H460(lane 1), HepG2(lane 2) cell line lysates (35ug/lane) |
2nd Image Caption |
Formalin-fixed and paraffin-embedded human hepatocarcinoma with VTN Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. |
3rd Image Caption |
Flow cytometric analysis of NCI-H292 cells using VTN Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.