Item no. |
PRS-62-572-400ul |
Manufacturer |
ProSci
|
Amount |
400 ul |
Category |
|
Type |
Antibody Polyclonal |
Format |
Liquid |
Applications |
IHC-P |
Specific against |
Human, Mouse |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
Lymphocyte activation gene 3 protein, LAG-3, Protein FDC, CD223, LAG3, FDC |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Immunology |
Immunogen |
This LAG3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 103-132 amino acids from the Central region of human LAG3. |
Applications |
For IHC-P starting dilution is: 1:25
For FACS starting dilution is: 1:25
For WB starting dilution is: 1:1000 |
Predicted Molecular Weight |
57 kDa |
Purification |
This antibody is purified through a protein A column, followed by peptide affinity purification. |
Clonality |
Polyclonal |
Isotype |
Rabbit Ig |
Conjugate |
Unconjugated |
Buffer |
Supplied in PBS with 0.09% (W/V) sodium azide. |
Concentration |
batch dependent |
Storage Conditions |
Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
LAG3 |
Accession # |
P18627 |
Protein Gi # |
251757512 |
Ncbi Gene Id # |
3902 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
LAG3 |
Ncbi Official Full Name |
Lymphocyte activation gene 3 protein |
Ncbi Organism |
Homo sapiens |
Swissprot # |
P18627 |
Background |
Lymphocyte-activation protein 3 belongs to Ig superfamily and contains 4 extracellular Ig-like domains. |
Background References 1 |
Smyth, D.J., et.al., BMC Med. Genet. 7, 20 (2006) |
1st Image Caption |
Antibody staining LAG3 in human thymus tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). |
2nd Image Caption |
Overlay histogram showing Jurkat cells stained with Antibody (red line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit lgG (H+L) (1583138) at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed. |
3rd Image Caption |
Western Blot at 1:1000 dilution Lane 1: human ovary lysate Lane 2: mouse liver lysate Lysates/proteins at 20 ug per lane. |
4th Image Caption |
Western Blot at 1:2000 dilution Lane 1: 293T/17 whole cell lysate Lane 2: mouse heart lysate Lysates/proteins at 20 ug per lane. |
5th Image Caption |
Western Blot at 1:2000 dilution + 293T/17 whole cell lysate Lysates/proteins at 20 ug per lane. |
6th Image Caption |
Western Blot at 1:2000 dilution + mouse heart lysate Lysates/proteins at 20 ug per lane. |
7th Image Caption |
Western Blot at 1:2000 dilution Lane 1: K562 whole cell lysates Lane 2: human liver lysates Lane 3: human ovary lysates Lysates/proteins at 20 ug per lane. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.