Comparison

HPGD Antibody

Item no. PRS-62-470-400ul
Manufacturer ProSci
Amount 400 ul
Category
Type Antibody Polyclonal
Format Liquid
Applications WB, FC, IHC-P
Specific against Human
Host Rabbit
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias 15-hydroxyprostaglandin dehydrogenase [NAD(+)], 15-PGDH, Prostaglandin dehydrogenase 1, HPGD, PGDH1
Available
Shipping
blue ice or RT
By Research Area
Immunology, Signal Transduction
Homology
Predicted species reactivity based on immunogen sequence: Monkey
Immunogen
This HPGD antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 184-212 amino acids from the C-terminal region of human HPGD.
Applications
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Predicted Molecular Weight
29 kDa
Purification
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality
Polyclonal
Isotype
Rabbit Ig
Conjugate
Unconjugated
Buffer
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration
batch dependent
Storage Conditions
Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Disclaimer
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications
None
Ncbi Official Symbol
HPGD
Accession #
P15428
Protein Gi #
129889
Ncbi Gene Id #
3248
User Note
Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol
HPGD
Ncbi Official Full Name
15-hydroxyprostaglandin dehydrogenase [NAD(+)]
Ncbi Organism
Homo sapiens
Swissprot #
P15428
Background
HPGD is a member of the short-chain nonmetalloenzyme alcohol dehydrogenase protein family. This protein is responsible for the metabolism of prostaglandins, which function in a variety of physiologic and cellular processes such as inflammation.
Background References 1
Thill, M., et.al., Anticancer Res. 29 (9), 3619-3625 (2009)
1st Image Caption
Western Blot at 1:2000 dilution + human placenta lysate Lysates/proteins at 20 ug per lane.
2nd Image Caption
Western blot analysis of HPGD Antibody in MDA-MB231 cell line lysates (35ug/lane)
3rd Image Caption
HPGD Antibody IHC analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
4th Image Caption
Flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 400 ul
Available: In stock
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