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KLF6 Antibody

KLF6 Antibody

Item no.	PRS-62-347-400ul
Manufacturer	ProSci
Amount	400 ul
Category	Neuroscience Neuroinflammation Antibodies Cell Biology Cell Status Apoptosis Flow Cytometry Immunohistochemistry Western Blot Immunofluorescence Primary Antibodies
Type	Antibody Polyclonal
Format	Liquid
Applications	WB, FC, IF, IHC-P
Specific against	Human, Mouse
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	Krueppel-like factor 6, B-cell-derived protein 1, Core promoter element-binding protein, GC-rich sites-binding factor GBF, Proto-oncogene BCD1, Suppressor of tumorigenicity 12 protein, Transcription factor Zf9, KLF6, BCD1, COPEB, CPBP, ST12
Available
Shipping	blue ice or RT
By Research Area	Signal Transduction
Immunogen	This KLF6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 159-186 amino acids from the C-terminal region of human KLF6.
Applications	For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50
Predicted Molecular Weight	32 kDa
Purification	This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality	Polyclonal
Isotype	Rabbit Ig
Conjugate	Unconjugated
Buffer	Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration	batch dependent
Storage Conditions	Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	KLF6
Accession #	Q99612
Protein Gi #	7387592
Ncbi Gene Id #	1316
User Note	Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol	KLF6
Ncbi Official Full Name	Krueppel-like factor 6
Ncbi Organism	Homo sapiens
Swissprot #	Q99612
Background	COPEB is a member of the Kruppel-like family of transcription factors. The zinc finger protein is a transcriptional activator, and functions as a tumor suppressor.
Background References 1	Sangodkar, J., Eur. J. Cancer 45 (4), 666-676 (2009) DiFeo, A., Drug Resist. Updat. 12 (1-2), 1-7 (2009)
1st Image Caption	Western blot analysis of KLF6 Antibody in mouse stomach tissue lysates (35ug/lane)
2nd Image Caption	Formalin-fixed and paraffin-embedded human testis tissue reacted with KLF6 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
3rd Image Caption	Flow cytometric analysis of widr cells using KLF6 Antibody (bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
4th Image Caption	Fluorescent confocal image of Hela cell stained with KLF6 Antibody .Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with KLF6 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). KLF6 immunoreactivity is localized to Nucleus significantly.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 400 ul

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Delivery expected until 9/6/2024