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AXIN2 Antibody

AXIN2 Antibody

Item no.	PRS-6163-0.02mg
Manufacturer	ProSci
Amount	0.02 mg
Category	Oncology Metabolism Cholesterol & Lipid Metabolism Antibodies Immunohistochemistry Western Blot ELISA Immunofluorescence Enzymes Other Fatty Acid Enzymes Primary Antibodies By Organ Colorectal Cancer Breast Cancer Nervous System Cancer
Type	Antibody Polyclonal
Format	Liquid
Applications	WB, IF, ELISA, IHC-P
Specific against	Human, Mouse, Rat
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	AXIN2 Antibody: AXIL, ODCRCS, Axin-2, Axin-like protein, Axil
Available
Shipping	blue ice or RT
By Research Area	Cancer, Cell Cycle
Homology	Predicted species reactivity based on immunogen sequence: Chicken: (85%)
Immunogen	AXIN2 antibody was raised against a 20 amino acid synthetic peptide near the carboxy terminus of human AXIN2. The immunogen is located within amino acids 780 - 830 of AXIN2.
Applications	AXIN2 antibody can be used for detection of AXIN2 by Western blot at 1 μ g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ g/mL. For immunofluorescence start at 20 μ g/mL. Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse and rat samples and Immunofluorescence in mouse and rat samples. All other applications and species not yet tested.
Specificity	AXIN2 antibody is predicted to not cross-react with AXIN1. At least four isoforms of AXIN2 are known to exist; this antibody will recognize all four.
Positive Control 1	Cat. No. 1402 - Mouse Lung Tissue Lysate
Predicted Molecular Weight	85, 93 kDa
Purification	AXIN2 Antibody is affinity chromatography purified via peptide column.
Clonality	Polyclonal
Isotype	IgG
Conjugate	Unconjugated
Buffer	AXIN2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration	1 mg/mL
Storage Conditions	AXIN2 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Related Products	Cat. No. 6163P – AXIN2 Peptide
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	AXIN2
Accession #	AAF22799
Protein Gi #	195927059
Ncbi Gene Id #	8313
User Note	Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol	AXIN2
Ncbi Official Full Name	axin 2
Ncbi Organism	Homo sapiens
Swissprot #	Q9Y2T1
Background	AXIN2 Antibody: Like the related protein AXIN1, AXIN2 is thought to play an important role in the regulation of the stability of beta-catenin in the Wnt signaling pathway. In mouse, AXIN2 organizes a multiprotein complex of APC (adenomatous polyposis of the colon), beta-catenin, glycogen synthase kinase 3-beta, and AXIN2, which leads to the degradation of beta-catenin, which is thought to be an important event in the genesis of a number of malignancies. The AXIN2 gene has been mapped to 17q23-q24, a region that shows frequent loss of heterozygosity in breast cancer, neuroblastoma, and other tumors. Mutations in this gene have been associated with colorectal cancer with defective mismatch repair.
Background References 1	Katoh M and Katoh M. WNT signaling pathway and stem cell signaling network. Clin. Cancer Res.2007; 13:4042-5.
Background References 2	Behrens J, Jerchow BA, Wurtele M, et al. Functional interaction of an axin homolog, conductin, with beta-catenin, APC, and GSK3beta. Science1998; 280:596-9.
Background References 3	Hadjihannas MV and Behrens J. CIN by WNT: growth pathways, mitotic control and chromosome instability in cancer. Cell Cycle2006; 5:2077-81.
Background References 4	Mai M, Qian C, Yokomizo A, et al. Cloning of the human homolog of conductin (AXIN2), a gene mapping to chromosome 17q23-q24. Genomics1999; 55:341-4.
1st Image Caption	Western blot analysis of AXIN2 in mouse lung lysate with AXIN2 antibody at 1 μ g/mL.
2nd Image Caption	Immunohistochemistry of AXIN2 in rat lung tissue with AXIN2 antibody at 5 μ g/mL.
3rd Image Caption	Immunofluorescence of AXIN2 in Rat Lung cells with AXIN2 antibody at 20 μ g/mL.
4th Image Caption	Immunohistochemistry of AXIN2 in mouse lung tissue with AXIN2 antibody at 5 μ g/mL.
5th Image Caption	Immunofluorescence of AXIN2 in mouse lung tissue with AXIN2 antibody at 20 μ g/mL. Red: AXIN2 Antibody (6163) Blue: DAPI staining
6th Image Caption	Immunofluorescence of AXIN2 in HepG2 cells with AXIN2 Antibody at 20 μ g/ml.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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