Item no. |
PRS-61-973-400ul |
Manufacturer |
ProSci
|
Amount |
400 ul |
Category |
|
Type |
Antibody Polyclonal |
Format |
Liquid |
Applications |
WB, IF, IHC-P |
Specific against |
Human |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
Vasopressin-neurophysin 2-copeptin, AVP-NPII, Arg-vasopressin, Arginine-vasopressin, Neurophysin 2, Neurophysin-II, Copeptin, AVP, ARVP, VP |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Neuroscience, Signal Transduction |
Immunogen |
This AVP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 102-129 amino acids from the Central region of human AVP. |
Applications |
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For IF starting dilution is: 1:10~50 |
Predicted Molecular Weight |
17 kDa |
Purification |
This antibody is purified through a protein A column, followed by peptide affinity purification. |
Clonality |
Polyclonal |
Isotype |
Rabbit Ig |
Conjugate |
Unconjugated |
Buffer |
Supplied in PBS with 0.09% (W/V) sodium azide. |
Concentration |
batch dependent |
Storage Conditions |
Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
AVP |
Accession # |
P01185 |
Protein Gi # |
128083 |
Ncbi Gene Id # |
551 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
AVP |
Ncbi Official Full Name |
Vasopressin-neurophysin 2-copeptin |
Ncbi Organism |
Homo sapiens |
Swissprot # |
P01185 |
Background |
This gene encodes a precursor protein consisting ofarginine vasopressin and two associated proteins, neurophysin 2 anda glycopeptide, copeptin. Arginine vasopressin is a posteriorpituitary hormone which is synthesized in the supraoptic nucleusand paraventricular nucleus of the hypothalamus. Along with itscarrier protein, neurophysin 2, it is packaged into neurosecretoryvesicles and transported axonally to the nerve endings in theneurohypophysis where it is either stored or secreted into thebloodstream. The precursor is thought to be activated while it isbeing transported along the axon to the posterior pituitary.Arginine vasopressin acts as a growth factor by enhancing pHregulation through acid-base transport systems. It has a directantidiuretic action on the kidney, and also causes vasoconstrictionof the peripheral vessels. This hormone can contract smooth muscleduring parturition and lactation. It is also involved in cognition, tolerance, adaptation and complex sexual and maternal behaviour, aswell as in the regulation of water excretion and cardiovascularfunctions. |
Background References 1 |
Abu Libdeh, A., et al. Eur. J. Endocrinol. 162(2):221-226(2010) |
Background References 2 |
Cirillo, M. Kidney Int. 77(1):5-6(2010) |
Background References 3 |
Meijer, E., et al. Kidney Int. 77(1):29-36(2010) |
Background References 4 |
Birk, J., et al. J. Cell. Sci. 122 (PT 21), 3994-4002 (2009) |
1st Image Caption |
Western Blot at 1:1000 dilution + ZR-75-1 whole cell lysate Lysates/proteins at 20 ug per lane. |
2nd Image Caption |
AVP Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. |
3rd Image Caption |
Confocal immunofluorescent analysis of AVP Antibody with ZR-75-1 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue). |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.