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DDAH1 Antibody

DDAH1 Antibody

Item no.	PRS-61-587-400ul
Manufacturer	ProSci
Amount	400 ul
Category	Oncology Neuroscience Neural Lineage Markers Molecular Targets for Neuroscience Antibodies Flow Cytometry Immunohistochemistry Western Blot Immunofluorescence Primary Antibodies By Organ Lung Cancer Testicular & Prostate Cancer Stem Cell Markers
Type	Antibody Polyclonal
Format	Liquid
Applications	WB, FC, IF, IHC-P
Specific against	Human, Mouse
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	N(G),N(G)-dimethylarginine dimethylaminohydrolase 1, DDAH-1, Dimethylarginine dimethylaminohydrolase 1, DDAHI, Dimethylargininase-1, DDAH1, DDAH
Available
Shipping	blue ice or RT
By Research Area	Neuroscience
Homology	Predicted species reactivity based on immunogen sequence: Bovine, Rat
Immunogen	This DDAH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 221-250 amino acids from the C-terminal region of human DDAH1.
Applications	For IHC-P starting dilution is: 1:50~100 For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50
Predicted Molecular Weight	31 kDa
Purification	This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality	Polyclonal
Isotype	Rabbit Ig
Conjugate	Unconjugated
Buffer	Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration	batch dependent
Storage Conditions	Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	DDAH1
Accession #	O94760
Ncbi Gene Id #	23576
User Note	Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol	DDAH1
Ncbi Official Full Name	N(G), N(G)-dimethylarginine dimethylaminohydrolase 1
Ncbi Organism	Homo sapiens
Swissprot #	O94760
Background	DDAH1 plays a role in nitric oxide generation by regulating cellular concentrations of methylarginines, which in turn inhibit nitric oxide synthase activity.
Background References 1	Ellger, B., et.al., Endocrinology 149 (6), 3148-3157 (2008)
1st Image Caption	Formalin-fixed and paraffin-embedded mouse kidney reacted with DDAH1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
2nd Image Caption	Western blot analysis of DDAH1 Antibody in mouse kidney tissue lysates (35ug/lane)
3rd Image Caption	Flow cytometric analysis of 293 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
4th Image Caption	Confocal immunofluorescent analysis of DDAH1 Antibody with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 400 ul

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Delivery expected until 9/6/2024

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