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LCK Antibody

LCK Antibody

Item no.	PRS-61-534-400ul
Manufacturer	ProSci
Amount	400 ul
Category	Oncology Neuroscience Neuroinflammation Aging & Neurological Disorders Protein Aggregation, Clearance & Misfolding Antibodies By Cell Type Carcinoma Sarcoma Flow Cytometry Immunohistochemistry Western Blot Primary Antibodies
Type	Antibody Polyclonal
Format	Liquid
Applications	WB, FC, IHC-P
Specific against	Human
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	Tyrosine-protein kinase Lck, Leukocyte C-terminal Src kinase, LSK, Lymphocyte cell-specific protein-tyrosine kinase, Protein YT16, Proto-oncogene Lck, T cell-specific protein-tyrosine kinase, p56-LCK, LCK
Available
Shipping	blue ice or RT
By Research Area	Immunology, Signal Transduction
Immunogen	This LCK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 480-509 amino acids from the C-terminal region of human LCK.
Applications	For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50
Predicted Molecular Weight	58 kDa
Purification	This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality	Polyclonal
Isotype	Rabbit Ig
Conjugate	Unconjugated
Buffer	Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration	batch dependent
Storage Conditions	Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	LCK
Accession #	P06239
Protein Gi #	125474
Ncbi Gene Id #	3932
User Note	Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol	LCK
Ncbi Official Full Name	Tyrosine-protein kinase Lck
Ncbi Organism	Homo sapiens
Swissprot #	P06239
Background	LCK is a member of the Src family of protein tyrosine kinases (PTKs). This protein is a key signaling molecule in the selection and maturation of developing T-cells. It contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. The protein localizes to the plasma membrane and pericentrosomal vesicles, and binds to cell surface receptors, including CD4 and CD8, and other signaling molecules.
Background References 1	Paster, W., J. Immunol. 182 (4), 2160-2167 (2009) Ngai, J., Eur. J. Immunol. 38 (11), 3208-3218 (2008) Strasner, A.B., J. Immunol. 181 (5), 3706-3713 (2008)
1st Image Caption	Western blot analysis in Ramos cell line lysates (35ug/lane).
2nd Image Caption	Formalin-fixed and paraffin-embedded human lymphoma reacted with LCK Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
3rd Image Caption	Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 400 ul

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Delivery expected until 9/6/2024

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