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U2AF1 Antibody

U2AF1 Antibody

Item no.	PRS-61-517-400ul
Manufacturer	ProSci
Amount	400 ul
Category	Neuroscience Neural Adhesion Molecules Antibodies Flow Cytometry Immunohistochemistry Western Blot Primary Antibodies
Type	Antibody Polyclonal
Format	Liquid
Applications	WB, FC, IHC-P
Specific against	Human
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	Splicing factor U2AF 35 kDa subunit, U2 auxiliary factor 35 kDa subunit, U2 small nuclear RNA auxiliary factor 1, U2 snRNP auxiliary factor small subunit, U2AF1, U2AF35, U2AFBP
Available
Shipping	blue ice or RT
Homology	Predicted species reactivity based on immunogen sequence: Bovine, Mouse
Immunogen	This U2AF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 99-125 amino acids from the Central region of human U2AF1.
Applications	For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50
Predicted Molecular Weight	28 kDa
Purification	This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality	Polyclonal
Isotype	Rabbit Ig
Conjugate	Unconjugated
Buffer	Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration	batch dependent
Storage Conditions	Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	U2AF1
Accession #	Q01081
Protein Gi #	267187
Ncbi Gene Id #	102724594, 7307
User Note	Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol	U2AF1
Ncbi Official Full Name	Splicing factor U2AF 35 kDa subunit
Ncbi Organism	Homo sapiens
Swissprot #	Q01081
Background	U2AF1 belongs to the splicing factor SR family. U2 auxiliary factor, comprising a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the small subunit which plays a critical role in both constitutive and enhancer-dependent RNA splicing by directly mediating interactions between the large subunit and proteins bound to the enhancers.
Background References 1	Rino, J., Mol. Cell. Biol. 28 (9), 3045-3057 (2008) Little, J.T., J. Biol. Chem. 283 (13), 8145-8152 (2008) Pacheco, T.R., Mol. Cell. Biol. 26 (21), 8183-8190 (2006)
1st Image Caption	Western blot analysis of anti- in Ramos cell line lysates (35ug/lane).
2nd Image Caption	Formalin-fixed and paraffin-embedded human lung carcinoma reacted with , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
3rd Image Caption	Flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
4th Image Caption	Western blot analysis of U2AF1 using rabbit polyclonal using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the U2AF1 gene.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 400 ul

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Delivery expected until 9/6/2024

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