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LGR5/GPR49 Antibody

LGR5/GPR49 Antibody

Item no.	PRS-61-473-400ul
Manufacturer	ProSci
Amount	400 ul
Category	Antibodies Cell Biology Cell Status Flow Cytometry Immunohistochemistry Western Blot Immunofluorescence Senescence Primary Antibodies
Type	Antibody Polyclonal
Format	Liquid
Applications	WB, FC, IF, IHC-P
Specific against	Human
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	Leucine-rich repeat-containing G-protein coupled receptor 5, G-protein coupled receptor 49, G-protein coupled receptor 67, G-protein coupled receptor HG38, LGR5, GPR49, GPR67
Available
Shipping	blue ice or RT
By Research Area	Cancer, Signal Transduction, Growth Factor
Immunogen	This LGR5/GPR49 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 238-270 amino acids from the N-terminal region of human LGR5/GPR49.
Applications	For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50
Predicted Molecular Weight	100 kDa
Purification	This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality	Polyclonal
Isotype	Rabbit Ig
Conjugate	Unconjugated
Buffer	Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration	batch dependent
Storage Conditions	Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	LGR5
Accession #	O75473
Ncbi Gene Id #	8549
User Note	Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol	LGR5
Ncbi Official Full Name	Leucine-rich repeat-containing G-protein coupled receptor 5
Ncbi Organism	Homo sapiens
Swissprot #	O75473
Background	LGR5/GPR49 is an orphan receptor. It may be an important receptor for signals controlling growth and differentiation of specific embryonic tissues.
Background References 1	Barker, N., Nature 449 (7165), 1003-1007 (2007)
Background References 2	McClanahan, T., Cancer Biol. Ther.(4), 419-426 (2006)
Background References 3	Yamamoto, Y., Hepatology 37 (3), 528-533 (2003)
Background References 4	Hsu, S.Y., Mol. Endocrinol. 14 (8), 1257-1271 (2000)
1st Image Caption	Western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the LGR5/GPR49 antibody detected the LGR5/GPR49 protein (arrow)(Kindly offered by Dr. Li).
2nd Image Caption	Formalin-fixed and paraffin-embedded human brain tissue reacted with LGR5/GPR49 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
3rd Image Caption	Flow cytometric analysis of HepG2 cells using LGR5/GPR49 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
4th Image Caption	Confocal immunofluorescent analysis of LGR5/GPR49 Antibody with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 400 ul

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Delivery expected until 10/4/2024

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