Item no. |
PRS-61-100-400ul |
Manufacturer |
ProSci
|
Amount |
400 ul |
Category |
|
Type |
Antibody Polyclonal |
Format |
Liquid |
Applications |
WB, FC |
Specific against |
Human |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
Ubiquitin carboxyl-terminal hydrolase 6, Deubiquitinating enzyme 6, Proto-oncogene TRE-2, Ubiquitin thioesterase 6, Ubiquitin-specific-processing protease 6, USP6, HRP1, TRE2 |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Cell Cycle |
Immunogen |
This USP6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1280-1310 amino acids from the C-terminal region of human USP6. |
Applications |
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50 |
Predicted Molecular Weight |
159 kDa |
Purification |
This antibody is purified through a protein A column, followed by peptide affinity purification. |
Clonality |
Polyclonal |
Isotype |
Rabbit Ig |
Conjugate |
Unconjugated |
Buffer |
Supplied in PBS with 0.09% (W/V) sodium azide. |
Concentration |
batch dependent |
Storage Conditions |
Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
USP6 |
Accession # |
P35125 |
Protein Gi # |
50403738 |
Ncbi Gene Id # |
9098 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
USP6 |
Ncbi Official Full Name |
Ubiquitin carboxyl-terminal hydrolase 6 |
Ncbi Organism |
Homo sapiens |
Swissprot # |
P35125 |
Background |
Modification of target proteins by ubiquitin participates in a wide array of biological functions. Proteins destined for degradation or processing via the 26 S proteasome are coupled to multiple copies of ubiquitin. However, attachment of ubiquitin or ubiquitin-related molecules may also result in changes in subcellular distribution or modification of protein activity. An additional level of ubiquitin regulation, deubiquitination, is catalyzed by proteases called deubiquitinating enzymes, which fall into four distinct families. Ubiquitin C-terminal hydrolases, ubiquitin-specific processing proteases (USPs), 1 OTU-domain ubiquitin-aldehyde-binding proteins, and Jab1/Pad1/MPN-domain-containing metallo-enzymes. Among these four families, USPs represent the most widespread and represented deubiquitinating enzymes across evolution. USPs tend to release ubiquitin from a conjugated protein. They display similar catalytic domains containing conserved Cys and His boxes but divergent N-terminal and occasionally C-terminal extensions, which are thought to function in substrate recognition, subcellular localization, and protein-protein interactions. |
Background References 1 |
Paulding, C.A., et al., Proc. Natl. Acad. Sci. U.S.A. 100(5):2507-2511 (2003). Papa, F.R., et al., Nature 366(6453):313-319 (1993). Nakamura, T., et al., Oncogene 7(4):733-741 (1992). |
1st Image Caption |
Western blot analysis in NCI-H460 cell line lysates (35ug/lane). |
2nd Image Caption |
Flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.