Item no. |
PRS-61-075-400ul |
Manufacturer |
ProSci
|
Amount |
400 ul |
Category |
|
Type |
Antibody Polyclonal |
Format |
Liquid |
Applications |
WB, IHC-P |
Specific against |
Human, Mouse |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
Ubiquitin conjugation factor E4 B, 632-, UBE4B (<a href="http://www.genenames.org/cgi-bin/gene_symbol_report?hgnc_id=12500" target="_blank">HGNC:12500</a>) |
Available |
|
Shipping |
blue ice or RT |
Immunogen |
This UBE4B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 112-142 amino acids from the N-terminal region of human UBE4B. |
Applications |
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100 |
Predicted Molecular Weight |
146 kDa |
Purification |
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis |
Clonality |
Polyclonal |
Isotype |
Rabbit Ig |
Conjugate |
Unconjugated |
Buffer |
Supplied in PBS with 0.09% (W/V) sodium azide. |
Concentration |
batch dependent |
Storage Conditions |
Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
UBE4B |
Accession # |
O95155 |
Ncbi Gene Id # |
10277 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
UBE4B |
Ncbi Official Full Name |
Ubiquitin conjugation factor E4 B |
Ncbi Organism |
Homo sapiens |
Swissprot # |
O95155 |
Background |
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE4B represents an additional conjugation factor, E4, which is involved in multiubiquitin chain assembly. UBE4B also the strongest candidate among the neuroblastoma tumor suppressors. |
Background References 1 |
Ishikawa, K., et al., DNA Res. 5(3):169-176 (1998). |
Background References 2 |
Mahoney, J.A., et al., Biochem. J. 361 (Pt 3), 587-595 (2002). |
1st Image Caption |
Antibody is used in Western blot to detect UBE4B in mouse kidney tissue lysate. |
2nd Image Caption |
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.