RARB Antibody

blue ice or RT

Predicted species reactivity based on immunogen sequence: Chicken, Mouse

For WB starting dilution is: 1:1000

For IF starting dilution is: 1:10~50

This antibody is purified through a protein A column, followed by peptide affinity purification.

Rabbit Ig

Supplied in PBS with 0.09% (W/V) sodium azide.

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

None

P10826

5915

RARB

Homo sapiens

This gene encodes retinoic acid receptor beta, a member ofthe thyroid-steroid hormone receptor superfamily of nucleartranscriptional regulators. This receptor localizes to thecytoplasm and to subnuclear compartments. It binds retinoic acid, the biologically active form of vitamin A which mediates cellularsignalling in embryonic morphogenesis, cell growth anddifferentiation. It is thought that this protein limits growth ofmany cell types by regulating gene expression. The gene was firstidentified in a hepatocellular carcinoma where it flanks ahepatitis B virus integration site. The gene expresses at least twotranscript variants; one additional transcript has been described, but its full length nature has not been determined. [provided byRefSeq].

Miladi-Abdennadher, I., et al. Tumour Biol. 31(5):503-511(2010)

Jugessur, A., et al. PLoS ONE(7), E11493 (2010) :

Fluorescent confocal image of Hela cell stained with RARB Antibody . Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with RARB primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). RARB immunoreactivity is localized to nucleus significantly.