Item no. |
PRS-59-680-400ul |
Manufacturer |
ProSci
|
Amount |
400 ul |
Category |
|
Type |
Antibody Polyclonal |
Format |
Liquid |
Applications |
WB, IF |
Specific against |
Human |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
Retinoic acid receptor beta, RAR-beta, HBV-activated protein, Nuclear receptor subfamily 1 group B member 2, RAR-epsilon, RARB, HAP, NR1B2 |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Cancer, Signal Transduction |
Homology |
Predicted species reactivity based on immunogen sequence: Chicken, Mouse |
Immunogen |
This RARB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 168-195 amino acids from the Central region of human RARB. |
Applications |
For WB starting dilution is: 1:1000
For IF starting dilution is: 1:10~50 |
Predicted Molecular Weight |
50 kDa |
Purification |
This antibody is purified through a protein A column, followed by peptide affinity purification. |
Clonality |
Polyclonal |
Isotype |
Rabbit Ig |
Conjugate |
Unconjugated |
Buffer |
Supplied in PBS with 0.09% (W/V) sodium azide. |
Concentration |
batch dependent |
Storage Conditions |
Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
RARB |
Accession # |
P10826 |
Protein Gi # |
17380507 |
Ncbi Gene Id # |
5915 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
RARB |
Ncbi Official Full Name |
Retinoic acid receptor beta |
Ncbi Organism |
Homo sapiens |
Swissprot # |
P10826 |
Background |
This gene encodes retinoic acid receptor beta, a member ofthe thyroid-steroid hormone receptor superfamily of nucleartranscriptional regulators. This receptor localizes to thecytoplasm and to subnuclear compartments. It binds retinoic acid, the biologically active form of vitamin A which mediates cellularsignalling in embryonic morphogenesis, cell growth anddifferentiation. It is thought that this protein limits growth ofmany cell types by regulating gene expression. The gene was firstidentified in a hepatocellular carcinoma where it flanks ahepatitis B virus integration site. The gene expresses at least twotranscript variants; one additional transcript has been described, but its full length nature has not been determined. [provided byRefSeq]. |
Background References 1 |
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010) |
Background References 2 |
Miladi-Abdennadher, I., et al. Tumour Biol. 31(5):503-511(2010) |
Background References 3 |
Ruano, G., et al. Pharmacogenomics 11(7):959-971(2010) |
Background References 4 |
Jugessur, A., et al. PLoS ONE(7), E11493 (2010) : |
1st Image Caption |
Western blot analysis in K562 cell line lysates (35ug/lane). |
2nd Image Caption |
Fluorescent confocal image of Hela cell stained with RARB Antibody . Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with RARB primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). RARB immunoreactivity is localized to nucleus significantly. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.