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RBBP8 Antibody

RBBP8 Antibody

Item no.	PRS-5761-0.1mg
Manufacturer	ProSci
Amount	0.1 mg
Category	Oncology Antibodies Immunohistochemistry Western Blot ELISA Immunofluorescence Primary Antibodies By Organ Breast Cancer Head & Neck Cancer
Type	Antibody Polyclonal
Format	Liquid
Applications	WB, IF, ELISA, IHC-P
Specific against	Human, Mouse, Rat
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	RBBP8 Antibody: RIM, COM1, CTIP, JWDS, SAE2, SCKL2, DNA endonuclease RBBP8, CtBP-interacting protein, CtIP
Available
Shipping	blue ice or RT
By Research Area	Cancer, Cell Cycle
Immunogen	RBBP8 antibody was raised against a 14 amino acid synthetic peptide near the center of human RBBP8. The immunogen is located within amino acids 430 - 480 of RBBP8.
Applications	RBBP8 antibody can be used for detection of RBBP8 by Western blot at 1 - 2 μ g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ g/mL. For immunofluorescence start at 20 μ g/mL. Antibody validated: Western Blot in mouse samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Positive Control 1	Cat. No. 1406 - Mouse Spleen Tissue Lysate
Purification	RBBP8 Antibody is affinity chromatography purified via peptide column.
Clonality	Polyclonal
Isotype	IgG
Conjugate	Unconjugated
Buffer	RBBP8 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration	1 mg/mL
Storage Conditions	RBBP8 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Related Products	Cat. No. 5761P – RBBP8 Peptide
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	RBBP8
Accession #	NP_976037
Protein Gi #	42718017
Ncbi Gene Id #	5932
User Note	Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol	RBBP8
Ncbi Official Full Name	retinoblastoma binding protein 8
Ncbi Organism	Homo sapiens
Swissprot #	Q99708
Background	RBBP8 Antibody: RBBP8, also known as CtBP (carboxy-terminal binding protein) interacting protein (CTIP), was characterized for its role in transcription as a cofactor for the transcriptional repressor CtBP, and also as a binding partner for other proteins including the cell cycle regulators retinoblastoma protein (Rb) and breast cancer 1 (BRCA1). It is ubiquitously expressed and localizes to the nucleus. RBBP8 is thought to modulate the functions in cell proliferation, transcriptional regulation and DNA repair. RBBP8 also plays a central role in the cell cycle checkpoint response to DNA double-stranded breaks (DSBs), with new evidence demonstrating that it controls the choice of DSB repair pathway.
Background References 1	Yu X, Wu LC, Bowcock AM, et al. The C-terminal (BRCT) domains of BRCA1 interact in vivo with CtIP, a protein implicated in the CtBP pathway of transcriptional repression. J. Biol. Chem.1998; 273:25388-92.
Background References 2	Liu F and Lee WH. CtIP activates its own and cyclin D1 promoters via the E2F/RB pathway during G1/S progression. Mol. Cell Biol.2006; 26:3124-34.
Background References 3	Sterner JM, Dew-Knight S, Musahl C, et al. Negative regulation of DNA replication by the retinoblastoma protein is mediated by its association with MCM7. Mol. Cell Biol.1998; 18:2748-57.
Background References 4	Wu G and Lee WH. CtIP, a multivalent adaptor connecting transcriptional regulation, checkpoint control and tumor suppression. Cell Cycle2006; 5:1592-6.
1st Image Caption	Western blot analysis of RBBP8 in mouse spleen tissue lysate with RBBP8 antibody at (A) 1 and (B) 2 μ g/mL.
2nd Image Caption	Immunohistochemistry of RBBP8 in rat spleen tissue with RBBP8 antibody at 5 μ g/mL.
3rd Image Caption	Immunofluorescence of RBBP8 in rat spleen tissue with RBBP8 antibody at 20 μ g/mL.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 0.1 mg

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Delivery expected until 9/6/2024

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