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HNMT Antibody

HNMT Antibody

Item no.	PRS-57-727-400ul
Manufacturer	ProSci
Amount	400 ul
Category	Oncology Neuroscience Neural Development Antibodies By Cell Type Blastoma Carcinoma Immunohistochemistry Western Blot Immunofluorescence Primary Antibodies
Type	Antibody Polyclonal
Format	Liquid
Applications	WB, IF, IHC-P
Specific against	Human
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	Histamine N-methyltransferase, HMT, HNMT
Available
Shipping	blue ice or RT
By Research Area	Neuroscience
Immunogen	This HNMT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 29-58 amino acids from the N-terminal region of human HNMT.
Applications	For IF starting dilution is: 1:10~50 For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight	33 kDa
Purification	This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality	Polyclonal
Isotype	Rabbit Ig
Conjugate	Unconjugated
Buffer	Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration	batch dependent
Storage Conditions	Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	HNMT
Accession #	P50135
Protein Gi #	1708272
Ncbi Gene Id #	3176
User Note	Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol	HNMT
Ncbi Official Full Name	Histamine N-methyltransferase
Ncbi Organism	Homo sapiens
Swissprot #	P50135
Background	In mammals, histamine is metabolized by two major pathways: N(tau)-methylation via histamine N-methyltransferase and oxidative deamination via diamine oxidase. HNMT is the first enzyme which is found in the cytosol and uses S-adenosyl-L-methionine as the methyl donor. In the mammalian brain, the neurotransmitter activity of histamine is controlled by N(tau)-methylation as diamine oxidase is not found in the central nervous system. A common genetic polymorphism affects the activity levels of this gene product in red blood cells.
Background References 1	Aksoy, S., et al., Biochem. Biophys. Res. Commun. 219(2):548-554 (1996).
1st Image Caption	Confocal immunofluorescent analysis of HNMT Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
2nd Image Caption	Western blot analysis of HNMT Antibody in Hela cell line lysates (35ug/lane). HNMT was detected using the purified polyclonal antibody.
3rd Image Caption	Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with HNMT antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 400 ul

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Delivery expected until 10/4/2024

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