Item no. |
PRS-57-727-400ul |
Manufacturer |
ProSci
|
Amount |
400 ul |
Category |
|
Type |
Antibody Polyclonal |
Format |
Liquid |
Applications |
WB, IF, IHC-P |
Specific against |
Human |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
Histamine N-methyltransferase, HMT, HNMT |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Neuroscience |
Immunogen |
This HNMT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 29-58 amino acids from the N-terminal region of human HNMT. |
Applications |
For IF starting dilution is: 1:10~50
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50 |
Predicted Molecular Weight |
33 kDa |
Purification |
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis |
Clonality |
Polyclonal |
Isotype |
Rabbit Ig |
Conjugate |
Unconjugated |
Buffer |
Supplied in PBS with 0.09% (W/V) sodium azide. |
Concentration |
batch dependent |
Storage Conditions |
Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
HNMT |
Accession # |
P50135 |
Protein Gi # |
1708272 |
Ncbi Gene Id # |
3176 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
HNMT |
Ncbi Official Full Name |
Histamine N-methyltransferase |
Ncbi Organism |
Homo sapiens |
Swissprot # |
P50135 |
Background |
In mammals, histamine is metabolized by two major pathways: N(tau)-methylation via histamine N-methyltransferase and oxidative deamination via diamine oxidase. HNMT is the first enzyme which is found in the cytosol and uses S-adenosyl-L-methionine as the methyl donor. In the mammalian brain, the neurotransmitter activity of histamine is controlled by N(tau)-methylation as diamine oxidase is not found in the central nervous system. A common genetic polymorphism affects the activity levels of this gene product in red blood cells. |
Background References 1 |
Aksoy, S., et al., Biochem. Biophys. Res. Commun. 219(2):548-554 (1996). |
1st Image Caption |
Confocal immunofluorescent analysis of HNMT Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue). |
2nd Image Caption |
Western blot analysis of HNMT Antibody in Hela cell line lysates (35ug/lane). HNMT was detected using the purified polyclonal antibody. |
3rd Image Caption |
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with HNMT antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.