Item no. |
PRS-57-567-400ul |
Manufacturer |
ProSci
|
Amount |
400 ul |
Category |
|
Type |
Antibody Polyclonal |
Format |
Liquid |
Applications |
WB, IHC-P |
Specific against |
Human, Mouse |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
RGM domain family member B, DRG11-responsive axonal guidance and outgrowth of neurite, DRAGON, RGMB |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Neuroscience, Signal Transduction |
Immunogen |
This DRAGON (RGMB) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 376-406 amino acids from the C-terminal region of human DRAGON (RGMB). |
Applications |
For WB starting dilution is: 1:2000
For IHC-P starting dilution is: 1:10~50 |
Predicted Molecular Weight |
48 kDa |
Purification |
This antibody is purified through a protein G column, followed by dialysis against PBS. |
Clonality |
Polyclonal |
Isotype |
Rabbit Ig |
Conjugate |
Unconjugated |
Buffer |
Supplied in PBS with 0.09% (W/V) sodium azide. |
Concentration |
batch dependent |
Storage Conditions |
Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
RGMB |
Accession # |
Q6NW40 |
Protein Gi # |
327478562 |
Ncbi Gene Id # |
285704 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
RGMB |
Ncbi Official Full Name |
RGM domain family member B |
Ncbi Organism |
Homo sapiens |
Swissprot # |
Q6NW40 |
Background |
Repulsive Guidance Molecule B (RGMB) is primarily expressed in the central nervous system. RGM A and B both appear at every level of the developing neural axis, where they colocalize to a large extent in the mantle layer, although only RGMA appears in the neuroepithelium, and only RGMB in the peripheral nervous system. During development, both RGMA and B appear also in lung and in limb cartilage, while RGMB has additional expression domains in pancreas. |
Background References 1 |
Schnichels, S., Gene Expr. Patterns(1), 1-11 (2007) |
Background References 2 |
Metzger, M., Dev. Dyn. 234 (1), 169-175 (2005) |
Background References 3 |
Xia, Y., Endocrinology 146 (8), 3614-3621 (2005) |
1st Image Caption |
Western Blot at 1:2000 dilution Lane 1: A431 whole cell lysate Lane 2: mouse brain lysate Lane 3: mouse cerebellum lysate Lane 4: NCI-H460 whole cell lysate Lysates/proteins at 20 ug per lane. |
2nd Image Caption |
Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with DRAGON (RGMB) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. |
3rd Image Caption |
Western blot analysis of DRAGON (RGMB) Antibody in K562 cell line lysates (35ug/lane) |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.