Item no. |
PRS-57-434-400ul |
Manufacturer |
ProSci
|
Amount |
400 ul |
Category |
|
Type |
Antibody Polyclonal |
Format |
Liquid |
Applications |
WB, IHC-P |
Specific against |
Human |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
Probable ATP-dependent RNA helicase DDX4, DEAD box protein 4, Vasa homolog, DDX4, VASA |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Cell Cycle, Signal Transduction |
Homology |
Predicted species reactivity based on immunogen sequence: Bovine, Monkey, Mouse, Pig, Rat |
Immunogen |
This DDX4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 554-583 amino acids from the C-terminal region of human DDX4. |
Applications |
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50 |
Predicted Molecular Weight |
79 kDa |
Purification |
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis |
Clonality |
Polyclonal |
Isotype |
Rabbit Ig |
Conjugate |
Unconjugated |
Buffer |
Supplied in PBS with 0.09% (W/V) sodium azide. |
Concentration |
batch dependent |
Storage Conditions |
Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
DDX4 |
Accession # |
Q9NQI0 |
Protein Gi # |
20138033 |
Ncbi Gene Id # |
54514 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
DDX4 |
Ncbi Official Full Name |
Probable ATP-dependent RNA helicase DDX4 |
Ncbi Organism |
Homo sapiens |
Swissprot # |
Q9NQI0 |
Background |
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. |
Background References 1 |
Guo, X., Asian J. Androl.(3), 339-344 (2007) |
Background References 2 |
Zeeman, A.M., Lab. Invest. 82 (2), 159-166 (2002) |
Background References 3 |
Castrillon, D.H., Proc. Natl. Acad. Sci. U.S.A. 97 (17), 9585-9590 (2000) |
1st Image Caption |
Western Blot at 1:1000 dilution + human ovary lysate Lysates/proteins at 20 ug per lane. |
2nd Image Caption |
Formalin-fixed and paraffin-embedded human testis tissue reacted with DDX4 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.