Comparison

DDX4 Antibody

Item no. PRS-57-434-400ul
Manufacturer ProSci
Amount 400 ul
Category
Type Antibody Polyclonal
Format Liquid
Applications WB, IHC-P
Specific against Human
Host Rabbit
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias Probable ATP-dependent RNA helicase DDX4, DEAD box protein 4, Vasa homolog, DDX4, VASA
Available
Shipping
blue ice or RT
By Research Area
Cell Cycle, Signal Transduction
Homology
Predicted species reactivity based on immunogen sequence: Bovine, Monkey, Mouse, Pig, Rat
Immunogen
This DDX4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 554-583 amino acids from the C-terminal region of human DDX4.
Applications
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight
79 kDa
Purification
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality
Polyclonal
Isotype
Rabbit Ig
Conjugate
Unconjugated
Buffer
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration
batch dependent
Storage Conditions
Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Disclaimer
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications
None
Ncbi Official Symbol
DDX4
Accession #
Q9NQI0
Protein Gi #
20138033
Ncbi Gene Id #
54514
User Note
Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol
DDX4
Ncbi Official Full Name
Probable ATP-dependent RNA helicase DDX4
Ncbi Organism
Homo sapiens
Swissprot #
Q9NQI0
Background
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division.
Background References 1
Guo, X., Asian J. Androl.(3), 339-344 (2007)
Background References 2
Zeeman, A.M., Lab. Invest. 82 (2), 159-166 (2002)
Background References 3
Castrillon, D.H., Proc. Natl. Acad. Sci. U.S.A. 97 (17), 9585-9590 (2000)
1st Image Caption
Western Blot at 1:1000 dilution + human ovary lysate Lysates/proteins at 20 ug per lane.
2nd Image Caption
Formalin-fixed and paraffin-embedded human testis tissue reacted with DDX4 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 400 ul
Available: In stock
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