Item no. |
PRS-57-268-400ul |
Manufacturer |
ProSci
|
Amount |
400 ul |
Category |
|
Type |
Antibody Polyclonal |
Format |
Liquid |
Applications |
WB, IHC-P |
Specific against |
Human |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
Non-POU domain-containing octamer-binding protein, NonO protein, 54 kDa nuclear RNA- and DNA-binding protein, 55 kDa nuclear protein, DNA-binding p52/p100 complex, 52 kDa subunit, NMT55, p54(nrb), p54nrb, NONO, NRB54 |
Available |
|
Shipping |
blue ice or RT |
Homology |
Predicted species reactivity based on immunogen sequence: Mouse, Rat |
Immunogen |
This NONO antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 45-74 amino acids from the N-terminal region of human NONO. |
Applications |
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50 |
Predicted Molecular Weight |
54 kDa |
Purification |
This antibody is purified through a protein A column, followed by peptide affinity purification. |
Clonality |
Polyclonal |
Isotype |
Rabbit Ig |
Conjugate |
Unconjugated |
Buffer |
Supplied in PBS with 0.09% (W/V) sodium azide. |
Concentration |
batch dependent |
Storage Conditions |
Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
NONO |
Accession # |
Q15233 |
Protein Gi # |
67469924 |
Ncbi Gene Id # |
4841 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
NONO |
Ncbi Official Full Name |
Non-POU domain-containing octamer-binding protein |
Ncbi Organism |
Homo sapiens |
Swissprot # |
Q15233 |
Background |
This gene encodes an RNA-binding protein which playsvarious roles in the nucleus, including transcriptional regulationand RNA splicing. A rearrangement between this gene and thetranscription factor E3 gene has been observed in papillary renalcell carcinoma. Alternatively spliced transcript variants have beendescribed. Pseudogenes exist on Chromosomes 2 and 16. [provided byRefSeq]. |
Background References 1 |
Marko, M., et al. Exp. Cell Res. 316(3):390-400(2010) |
Background References 2 |
Dong, X., et al. Mol. Endocrinol. 23(8):1147-1160(2009) |
Background References 3 |
Sikora, D., et al. Virology 390(1):71-78(2009) |
Background References 4 |
Clemson, C.M., et al. Mol. Cell 33(6):717-726(2009) |
1st Image Caption |
Western blot analysis in Hela cell line lysates (35ug/lane). |
2nd Image Caption |
NONO Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.