Item no. |
PRS-5697-0.02mg |
Manufacturer |
ProSci
|
Amount |
0.02 mg |
Category |
|
Type |
Antibody Polyclonal |
Format |
Liquid |
Applications |
WB, IF, ICC, ELISA |
Specific against |
Human, Mouse, Rat |
Host |
Chicken |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
FAM82A1 Antibody: RMD2, RMD4, RMD-2, FAM82A, BLOCK18, FAM82A1, PRO34163, PYST9371, UNQ9371/PRO34163, Regulator of microtubule dynamics protein 2, Protein FAM82A1 |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Homeostasis |
Homology |
Predicted species reactivity based on immunogen sequence: Bovine: (94%) |
Immunogen |
FAM82A1 antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human FAM82A1.
The immunogen is located within amino acids 140 - 190 of FAM82A1. |
Applications |
FAM82A1 antibody can be used for detection of FAM82A1 by Western blot at 1 - 2 μ g/mL. Antibody can also be used for immunocytochemistry starting at 5 μ g/mL. For immunofluorescence start at 20 μ g/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. |
Positive Control 1 |
Cat. No. 1204 - K562 Cell Lysate |
Positive Control 2 |
Cat. No. 17-005 - Jurkat Cell Slide |
Positive Control 3 |
Cat. No. 17-004 - K-562 Cell Slide |
Purification |
FAM82A1 Antibody is affinity chromatography purified via peptide column. |
Clonality |
Polyclonal |
Isotype |
IgY |
Conjugate |
Unconjugated |
Buffer |
FAM82A1 Antibody is supplied in PBS containing 0.02% sodium azide. |
Concentration |
1 mg/mL |
Storage Conditions |
FAM82A1 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Related Products |
Cat. No. 5697P – FAM82A1 Peptide |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
FAM82A1 |
Accession # |
NP_653314 |
Protein Gi # |
283046686 |
Ncbi Gene Id # |
151393 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
FAM82A1 |
Ncbi Official Full Name |
family with sequence similarity 82, member A1 |
Ncbi Organism |
Homo sapiens |
Swissprot # |
Q96LZ7 |
Background |
FAM82A1 Antibody: The human protein FAM82A1, also known as regulator of microtubule dynamics 2 (RMD2), was one of three proteins identified as related to a family of microtubule-associated proteins in C. elegans. FAM82A1 contains multiple coiled-coil domains and localize to the spindle microtubules and spindle poles during cell division. During interphase, RMD2 localizes to the cytoplasm with protein observed in the microtubule lattice and perinuclear region. |
Background References 1 |
Oishi K, Okanu H, and Sawa H. RMD-1, a novel microtubule-associated protein, functions in chromosome segregation in Caenorhabditis elegans. J. Cell Biol.2007; 179:1149-62. |
1st Image Caption |
Western blot analysis of FAM82A1 in K562 cell lysate with FAM82A1 antibody at (A) 1 and (B) 2 μ g/mL. |
2nd Image Caption |
Immunocytochemistry of FAM82A1 in Jurkat cells with ENC-2 antibody at 5 μ g/mL. |
3rd Image Caption |
Immunofluorescence of FAM82A1 in K562 cells with FAM82A1 antibody at 20 μ g/mL. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.