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NSA1 Antibody

NSA1 Antibody

Item no.	PRS-5669-0.1mg
Manufacturer	ProSci
Amount	0.1 mg
Category	Antibodies Immunohistochemistry Western Blot ELISA Immunofluorescence Primary Antibodies
Type	Antibody Polyclonal
Format	Liquid
Applications	WB, IF, ELISA, IHC-P
Specific against	Human, Mouse, Rat
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	NSA1 Antibody: NSA1, WD repeat-containing protein 74, NOP seven-associated protein 1
Available
Shipping	blue ice or RT
By Research Area	Cancer, Cell Cycle
Homology	Predicted species reactivity based on immunogen sequence: Bovine: (94%)
Immunogen	NSA1 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human NSA1. The immunogen is located within amino acids 320 - 370 of NSA1.
Applications	NSA1 antibody can be used for detection of NSA1 by Western blot at 1 - 2 μ g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ g/mL. For immunofluorescence start at 20 μ g/mL. Antibody validated: Western Blot in mouse samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Positive Control 1	Cat. No. 1404 - Mouse Liver Tissue Lysate
Purification	NSA1 Antibody is affinity chromatography purified via peptide column.
Clonality	Polyclonal
Isotype	IgG
Conjugate	Unconjugated
Buffer	NSA1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration	1 mg/mL
Storage Conditions	NSA1 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Related Products	Cat. No. 5669P – NSA1 Peptide
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	WDR74
Accession #	Q6RFH5
Protein Gi #	55976441
Ncbi Gene Id #	54663
User Note	Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol	WDR74
Ncbi Official Full Name	WD repeat domain 74
Ncbi Organism	Homo sapiens
Swissprot #	Q6RFH5
Background	NSA1 Antibody: The yeast nucleolar protein NOP7 is necessary for the maturation of 66S preribosomes and interacts with numerous other proteins. One such protein is an essential, conserved WD repeat protein, NOP seven-associated protein 1 (NSA1), that is also required for the yeast 66S ribosome assembly. NSA1 is also associated with the AAA ATPase Rix7, and release of NSA1 from a novel late nucleolar pre-60S requires the Rix7 function. NSA1 has also been found upregulated in mammalian cancer cells, suggesting it may also play a role in cell proliferation.
Background References 1	Miles TD, Jakovljevic J, Horsey EW, et al. Ytm1, Nop7, and Erb1 form a complex necessary for maturation of yeast 66S preribosomes. Mol. Cell Biol.2005; 25:10419-32.
Background References 2	Harnpicharnchai P, Jakovljevic J, Horsey E, et al. Composition and functional characterization of yeast 66S ribosome assembly intermediates. Mol. Cell2001; 8:505-15.
Background References 3	Kressler D, Roser D, Pertschy B, et al. The AAA ATPase Rix7 powers progression of ribosome biogenesis by stripping Nsa1 from pre-60S particles. J. Cell Biol.2008; 181:935-44.
Background References 4	Krol M, Polanska J, Pawlowski KM, et al. Transcriptomic signature of cell lines isolated from canine mammary adenocarcinoma metastases to lungs. J. Appl. Genet.2010; 51:37-50.
1st Image Caption	Western blot analysis of NSA1 in mouse liver tissue lysate with NSA1 antibody at (A) 1 and (B) 2 μ g/mL.
2nd Image Caption	Immunohistochemistry of NSA1 in rat liver tissue with NSA1 antibody at 5 μ g/mL.
3rd Image Caption	Immunofluorescence of NSA1 in rat liver tissue with NSA1 antibody at 20 μ g/mL.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 0.1 mg

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