Item no. |
PRS-5647-0.1mg |
Manufacturer |
ProSci
|
Amount |
0.1 mg |
Category |
|
Type |
Antibody Polyclonal |
Format |
Liquid |
Applications |
WB, IF, ELISA, IHC-P |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
Slc37A2 Antibody: pp11662, SPX2, Sugar phosphate exchanger 2, Solute carrier family 37 member 2 |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Obesity |
Immunogen |
Slc37A2 antibody was raised against a 15 amino acid synthetic peptide near the center of human Slc37A2.
The immunogen is located within amino acids 230 - 280 of Slc37A2. |
Applications |
Slc37A2 antibody can be used for detection of Slc37A2 by Western blot at 1 μ g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ g/mL. For immunofluorescence start at 20 μ g/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested. |
Positive Control 1 |
Cat. No. 1406 - Mouse Spleen Tissue Lysate |
Purification |
Slc37A2 Antibody is affinity chromatography purified via peptide column. |
Clonality |
Polyclonal |
Isotype |
IgG |
Conjugate |
Unconjugated |
Buffer |
Slc37A2 Antibody is supplied in PBS containing 0.02% sodium azide. |
Concentration |
1 mg/mL |
Storage Conditions |
Slc37A2 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Related Products |
Cat. No. 5647P – Slc37A2 Peptide |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
SLC37A2 |
Accession # |
NP_938018 |
Protein Gi # |
38093649 |
Ncbi Gene Id # |
219855 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
SLC37A2 |
Ncbi Official Full Name |
solute carrier family 37 (glycerol-3-phosphate transporter), member 2 |
Ncbi Organism |
Homo sapiens |
Swissprot # |
Q8TED4 |
Background |
Slc37A2 Antibody: The Slc37 gene family consists of four members which have 10-12 predicted transmembrane domains and belong to the sugar-phosphate transporter superfamily. Recent experiments have demonstrated that Slc37A2 is a macrophage-specific gene selectively expressed in obese white adipose tissue. Its transcript is also expressed in spleen and thymus and the cell lines RAW264.7 and THP-1. Compared to wild-type mice, Slc37A2 mRNA is expressed at nine-fold higher levels in abdominal epididymal fat deposits of ob/ob mice, suggesting that it may be required to limit the contribution of macrophages to the inflammatory state present in obesity. Slc37A2 protein is post-translationally modified with the addition of N-linked glycans and migrates as a heterogeneous species of 50-75kDa. |
Background References 1 |
Bartolini L and Antonarakis SE. The human sugar-phosphate/phosphate exchanger family SLC37. Eur. J. Physiol.2004; 447-780-3. |
Background References 2 |
Kim JY, Tillison K, Zhou S, et al. The major facilitator superfamily member Slc37a2 is a novel macrophage-specific gene selectively expressed in obese white adipose tissue. Am. J. Physiol. Endocrinol. Metab.2007; 293:E110-20. |
1st Image Caption |
Western blot analysis of Slc37A2 in mouse spleen tissue lysate with Slc37A2 antibody at 1 μ g/mL in the (A) absence and (B) presence of blocking peptide. |
2nd Image Caption |
Immunohistochemistry of Slc37A2 in rat spleen tissue with Slc37A2 antibody at 5 μ g/mL. |
3rd Image Caption |
Immunofluorescence of Slc37A2 in rat spleen tissue with Slc37A2 antibody at 20 μ g/mL. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.