Item no. |
PRS-56-976-400ul |
Manufacturer |
ProSci
|
Amount |
400 ul |
Category |
|
Type |
Antibody Polyclonal |
Format |
Liquid |
Applications |
WB, IHC-P |
Specific against |
Human |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27, p27Kip1, CDKN1B, KIP1 |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Cancer, Cell Cycle |
Immunogen |
This CDKN1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 147-176 amino acids from the C-terminal region of human CDKN1B. |
Applications |
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50 |
Predicted Molecular Weight |
22 kDa |
Purification |
This antibody is purified through a protein A column, followed by peptide affinity purification. |
Clonality |
Polyclonal |
Isotype |
Rabbit Ig |
Conjugate |
Unconjugated |
Buffer |
Supplied in PBS with 0.09% (W/V) sodium azide. |
Concentration |
batch dependent |
Storage Conditions |
Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
CDKN1B |
Accession # |
P46527 |
Ncbi Gene Id # |
1027 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
CDKN1B |
Ncbi Official Full Name |
Cyclin-dependent kinase inhibitor 1B |
Ncbi Organism |
Homo sapiens |
Swissprot # |
P46527 |
Background |
This gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. [provided by RefSeq]. |
Background References 1 |
Kajihara, R., et al. Biochem. Biophys. Res. Commun. 401(3):350-355(2010) Kedde, M., et al. Nat. Cell Biol. 12(10):1014-1020(2010) Canbay, E., et al. Anticancer Res. 30(7):3093-3098(2010) Do Nascimento Borges, B., et al. In Vivo 24(4):579-582(2010) Qin, J., et al. Hepatogastroenterology 57 (99-100), 547-553 (2010) : |
1st Image Caption |
Western blot analysis in human normal Uterus tissue lysates (35ug/lane). |
2nd Image Caption |
CDKN1B Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.