Item no. |
PRS-56-188-400ul |
Manufacturer |
ProSci
|
Amount |
400 ul |
Category |
|
Type |
Antibody Polyclonal |
Format |
Liquid |
Applications |
WB, IF, IHC-P |
Specific against |
Human, Mouse |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
Segment polarity protein dishevelled homolog DVL-3, Dishevelled-3, DSH homolog 3, DVL3, KIAA0208 |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Neuroscience, Signal Transduction |
Immunogen |
This DVL3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 530-557 amino acids from the C-terminal region of human DVL3. |
Applications |
For WB starting dilution is: 1:2000
For IHC-P starting dilution is: 1:10~50
For IF starting dilution is: 1:10~50 |
Predicted Molecular Weight |
78 kDa |
Purification |
This antibody is purified through a protein A column, followed by peptide affinity purification. |
Clonality |
Polyclonal |
Isotype |
Rabbit Ig |
Conjugate |
Unconjugated |
Buffer |
Supplied in PBS with 0.09% (W/V) sodium azide. |
Concentration |
batch dependent |
Storage Conditions |
Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
DVL3 |
Accession # |
Q92997 |
Protein Gi # |
6919875 |
Ncbi Gene Id # |
1857 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
DVL3 |
Ncbi Official Full Name |
Segment polarity protein dishevelled homolog DVL-3 |
Ncbi Organism |
Homo sapiens |
Swissprot # |
Q92997 |
Background |
This gene is a member of a multi-gene family which sharesstrong similarity with the Drosophila dishevelled gene, dsh. TheDrosophila dishevelled gene encodes a cytoplasmic phosphoproteinthat regulates cell proliferation. |
Background References 1 |
Gnad, T., et al. Mol. Cancer 9, 31 (2010) : |
Background References 2 |
Bouteille, N., et al. Oncogene 28(28):2569-2580(2009) |
Background References 3 |
Fukukawa, C., et al. Oncogene 28(8):1110-1120(2009) |
Background References 4 |
Wei, Q., et al. Lung Cancer 62(2):181-192(2008) |
1st Image Caption |
Western Blot at 1:2000 dilution Lane 1: A549 whole cell lysates Lane 2: NIH/3T3 whole cell lysates Lane 3: K562 whole cell lysates Lysates/proteins at 20 ug per lane. |
2nd Image Caption |
DVL3 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. |
3rd Image Caption |
Confocal immunofluorescent analysis of DVL3 Antibody with A375 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue). |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.