Item no. |
PRS-55-715-400ul |
Manufacturer |
ProSci
|
Amount |
400 ul |
Category |
|
Type |
Antibody Polyclonal |
Format |
Liquid |
Applications |
WB, IHC-P |
Specific against |
Human, Mouse |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
Translation initiation factor eIF-2B subunit delta, eIF-2B GDP-GTP exchange factor subunit delta, EIF2B4, EIF2BD |
Available |
|
Shipping |
blue ice or RT |
Homology |
Predicted species reactivity based on immunogen sequence: Bovine |
Immunogen |
This EIF2B4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 146-175 amino acids from the Central region of human EIF2B4. |
Applications |
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100 |
Predicted Molecular Weight |
58 kDa |
Purification |
This antibody is purified through a protein A column, followed by peptide affinity purification. |
Clonality |
Polyclonal |
Isotype |
Rabbit Ig |
Conjugate |
Unconjugated |
Buffer |
Supplied in PBS with 0.09% (W/V) sodium azide. |
Concentration |
batch dependent |
Storage Conditions |
Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
EIF2B4 |
Accession # |
Q9UI10 |
Protein Gi # |
28381357 |
Ncbi Gene Id # |
8890 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
EIF2B4 |
Ncbi Official Full Name |
Translation initiation factor eIF-2B subunit delta |
Ncbi Organism |
Homo sapiens |
Swissprot # |
Q9UI10 |
Background |
Eukaryotic initiation factor 2B (EIF2B), which isnecessary for protein synthesis, is a GTP exchange factor composedof five different subunits. The protein encoded by this gene is thefourth, or delta, subunit. Defects in this gene are a cause ofleukoencephalopathy with vanishing white matter (VWM) andovarioleukodystrophy. Multiple transcript variants encodingdifferent isoforms have been found for this gene. [provided byRefSeq]. |
Background References 1 |
Naukkarinen, J., et al. PLoS Genet.(6), E1000976 (2010) : |
Background References 2 |
Wu, Y., et al. J. Hum. Genet. 54(2):74-77(2009) |
Background References 3 |
Horzinski, L., et al. PLoS ONE(12), E8318 (2009) : |
Background References 4 |
Pronk, J., et al. Mult. Scler. 14(8):1123-1126(2008) |
1st Image Caption |
Western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the EIF2B4 antibody detected the EIF2B4 protein (arrow). |
2nd Image Caption |
Western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the EIF2B4 antibody detected the EIF2B4 protein (arrow). |
3rd Image Caption |
E immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of E for immunohistochemistry. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.