HP Antibody

Cell Cycle, Signal Transduction

For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50

For IF starting dilution is: 1:10~50

This antibody is purified through a protein A column, followed by peptide affinity purification.

Rabbit Ig

Supplied in PBS with 0.09% (W/V) sodium azide.

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

None

P00738

3240

HP

Homo sapiens

This gene encodes a preproprotein, which is processed toyield both alpha and beta chains, which subsequently combine as atetramer to produce haptoglobin. Haptoglobin functions to bind freeplasma hemoglobin, which allows degradative enzymes to gain accessto the hemoglobin, while at the same time preventing loss of ironthrough the kidneys and protecting the kidneys from damage byhemoglobin. Mutations in this gene and/or its regulatory regionscause ahaptoglobinemia or hypohaptoglobinemia. This gene has alsobeen linked to diabetic nephropathy, the incidence of coronaryartery disease in type 1 diabetes, Crohn's disease, inflammatorydisease behavior, primary sclerosing cholangitis, susceptibility toidiopathic Parkinson's disease, and a reduced incidence ofPlasmodium falciparum malaria. A similar duplicated gene is locatednext to this gene on chromosome 16. Multiple transcript variantsencoding different isoforms have been found for this gene.

Ucisik-Akkaya, E., et al. Mol. Hum. Reprod. 16(10):770-777(2010)

Savy, M., et al. PLoS ONE(6), E11075 (2010) :

Western blot analysis of HP using rabbit polyclonal HP Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HP gene.

Flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis in MDA-MB453, CEM, NCI-H460, HepG2, K562 cell line lysates (35ug/lane).This demonstrates the detected the HP protein (arrow).