Item no. |
PRS-55-226-400ul |
Manufacturer |
ProSci
|
Amount |
400 ul |
Category |
|
Type |
Antibody Polyclonal |
Format |
Liquid |
Applications |
WB, FC, IHC-P |
Specific against |
Human |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
Vitrin, VIT |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Signal Transduction |
Homology |
Predicted species reactivity based on immunogen sequence: Bovine, Mouse |
Immunogen |
This VITRN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 567-595 amino acids from the C-terminal region of human VITRN. |
Applications |
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50 |
Predicted Molecular Weight |
74 kDa |
Purification |
This antibody is purified through a protein A column, followed by peptide affinity purification. |
Clonality |
Polyclonal |
Isotype |
Rabbit Ig |
Conjugate |
Unconjugated |
Buffer |
Supplied in PBS with 0.09% (W/V) sodium azide. |
Concentration |
batch dependent |
Storage Conditions |
Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
VIT |
Accession # |
Q6UXI7 |
Protein Gi # |
74739159 |
Ncbi Gene Id # |
5212 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
VIT |
Ncbi Official Full Name |
Vitrin |
Ncbi Organism |
Homo sapiens |
Swissprot # |
Q6UXI7 |
Background |
Promotes matrix assembly and cell adhesiveness (By similarity). |
Background References 1 |
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) : |
Background References 2 |
Clark, H.F., et al. Genome Res. 13(10):2265-2270(2003) |
Background References 3 |
Tomarev, S.I., et al. Invest. Ophthalmol. Vis. Sci. 44(6):2588-2596(2003) |
Background References 4 |
Whittaker, C.A., et al. Mol. Biol. Cell 13(10):3369-3387(2002) |
1st Image Caption |
Western blot analysis in U251 cell line lysates (35ug/lane). |
2nd Image Caption |
VITRN antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. |
3rd Image Caption |
Flow cytometric analysis of U251 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.