Item no. |
PRS-55-204-400ul |
Manufacturer |
ProSci
|
Amount |
400 ul |
Category |
|
Type |
Antibody Polyclonal |
Format |
Liquid |
Applications |
WB, IHC-P |
Specific against |
Human, Mouse |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
Lysosomal protective protein, Carboxypeptidase C, Carboxypeptidase L, Cathepsin A, Protective protein cathepsin A, PPCA, Protective protein for beta-galactosidase, Lysosomal protective protein 32 kDa chain, Lysosomal protective protein 20 kDa chain, CTSA, |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Obesity, Signal Transduction |
Immunogen |
This CTSA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 18-45 amino acids from the N-terminal region of human CTSA. |
Applications |
For IHC-P starting dilution is: 1:25
For WB starting dilution is: 1:1000 |
Predicted Molecular Weight |
54 kDa |
Purification |
This antibody is purified through a protein A column, followed by peptide affinity purification. |
Clonality |
Polyclonal |
Isotype |
Rabbit Ig |
Conjugate |
Unconjugated |
Buffer |
Supplied in PBS with 0.09% (W/V) sodium azide. |
Concentration |
batch dependent |
Storage Conditions |
Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
CTSA |
Accession # |
P10619 |
Protein Gi # |
20178316 |
Ncbi Gene Id # |
5476 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
CTSA |
Ncbi Official Full Name |
Lysosomal protective protein |
Ncbi Organism |
Homo sapiens |
Swissprot # |
P10619 |
Background |
CTSA encodes a glycoprotein which associates withlysosomal enzymes beta-galactosidase and neuraminidase to form acomplex of high molecular weight multimers. The formation of thiscomplex provides a protective role for stability and activity.Deficiencies in this gene are linked to multiple forms ofgalactosialidosis. |
Background References 1 |
Reich, M., et al. Immunol. Lett. 128(2):143-147(2010) |
Background References 2 |
Bonten, E.J., et al. J. Biol. Chem. 284(41):28430-28441(2009) |
Background References 3 |
Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) : |
Background References 4 |
Tatano, Y., et al. J. Med. Invest. 53 (1-2), 103-112 (2006) : |
1st Image Caption |
Immunohistochemical analysis of paraffin-embedded H. kidney section using CTSA Antibody (N-term). Antibody was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining. |
2nd Image Caption |
Western blot analysis of lysates from A431, Hela, HepG2, HT-1080 cell line and human placenta, kidney tissue lysate (from left to right), using CTSA Antibody at 1:1000 at each lane. |
3rd Image Caption |
Western blot analysis in HepG2 cell line lysates (35ug/lane). |
4th Image Caption |
Western blot analysis in mouse bladder tissue lysates (35ug/lane). |
5th Image Caption |
CTSA antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.