Item no. |
PRS-55-132-400ul |
Manufacturer |
ProSci
|
Amount |
400 ul |
Category |
|
Type |
Antibody Polyclonal |
Format |
Liquid |
Applications |
WB |
Specific against |
Human |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
DNA (cytosine-5)-methyltransferase 1, Dnmt1, CXXC-type zinc finger protein 9, DNA methyltransferase HsaI, DNA MTase HsaI, MHsaI, MCMT, DNMT1, AIM, CXXC9, DNMT |
Available |
|
Shipping |
blue ice or RT |
Homology |
Predicted species reactivity based on immunogen sequence: Mouse, Rat |
Immunogen |
This DNMT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1083-1112 amino acids from human DNMT1. |
Applications |
For WB starting dilution is: 1:1000 |
Predicted Molecular Weight |
183 kDa |
Purification |
This antibody is purified through a protein A column, followed by peptide affinity purification. |
Clonality |
Polyclonal |
Isotype |
Rabbit Ig |
Conjugate |
Unconjugated |
Buffer |
Supplied in PBS with 0.09% (W/V) sodium azide. |
Concentration |
batch dependent |
Storage Conditions |
Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
DNMT1 |
Accession # |
P26358 |
Protein Gi # |
12231019 |
Ncbi Gene Id # |
1786 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
DNMT1 |
Ncbi Official Full Name |
DNA (cytosine-5)-methyltransferase 1 |
Ncbi Organism |
Homo sapiens |
Swissprot # |
P26358 |
Background |
Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Dnmt1 co-purifies with the retinoblastoma (Rb) tumour suppressor gene product, E2F1, and HDAC1. Dnmt1 also cooperates with Rb to repress transcription from promoters containing E2Fbinding sites suggesting a link between DNA methylation, histone deacetylase and sequence-specific DNA binding activity, as well as a growth-regulatory pathway that is disrupted in nearly all cancer cells. |
Background References 1 |
Peterson, E.J., et al., Cancer Res. 63(20):6579-6582 (2003). |
Background References 2 |
Leu, Y.W., et al., Cancer Res. 63(19):6110-6115 (2003). |
Background References 3 |
Saito, Y., et al., Int. J. Cancer 105(4):527-532 (2003). |
Background References 4 |
Siedlecki, P., et al., Biochem. Biophys. Res. Commun. 306(2):558-563 (2003). |
1st Image Caption |
Western blot analysis of DNMT1 Antibody (S1105) in MDA-MB435 cell line lysates (35ug/lane) |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.