Item no. |
PRS-55-092-400ul |
Manufacturer |
ProSci
|
Amount |
400 ul |
Category |
|
Type |
Antibody Polyclonal |
Format |
Liquid |
Applications |
WB, FC, IHC-P |
Specific against |
Human |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
Gamma-glutamyltransferase light chain 2, Gamma-glutamyltransferase-like protein 4, GGTLC2, GGTL4 |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Obesity, Signal Transduction |
Homology |
Predicted species reactivity based on immunogen sequence: Mouse, Pig, Rat |
Immunogen |
This GGTLC2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 127-154 amino acids from the Central region of human GGTLC2. |
Applications |
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50 |
Predicted Molecular Weight |
24 kDa |
Purification |
This antibody is purified through a protein A column, followed by peptide affinity purification. |
Clonality |
Polyclonal |
Isotype |
Rabbit Ig |
Conjugate |
Unconjugated |
Buffer |
Supplied in PBS with 0.09% (W/V) sodium azide. |
Concentration |
batch dependent |
Storage Conditions |
Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
GGTLC2 |
Accession # |
Q14390 |
Protein Gi # |
294862536 |
Ncbi Gene Id # |
91227 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
GGTLC2 |
Ncbi Official Full Name |
Gamma-glutamyltransferase light chain 2 |
Ncbi Organism |
Homo sapiens |
Swissprot # |
Q14390 |
Background |
Gamma-glutamyltransferase-1 (GGT1; MIM 612346) is amembrane-bound extracellular enzyme that cleaves gamma-glutamylpeptide bonds in glutathione and other peptides and transfers thegamma-glutamyl moiety to acceptors. Autocatalytic cleavage of theGGT1 precursor polypeptide produces a heavy chain and a light chainthat associate with each other to form the functional enzyme. Lightchain-only GGTs, such as GGTLC2, contain a region corresponding tothe GGT1 light chain, but they lack the membrane-anchoring heavychain region (Heisterkamp et al., 2008 [PubMed 18357469]).[suppliedby OMIM]. |
Background References 1 |
Heisterkamp, N., et al. Hum. Genet. 123(4):321-332(2008) |
Background References 2 |
Dunham, I., et al. Nature 402(6761):489-495(1999) |
Background References 3 |
Kawasaki, K., et al. Genome Res. 7(3):250-261(1997) |
Background References 4 |
Leh, H., et al. FEBS Lett. 394(3):258-262(1996) |
1st Image Caption |
Western blot analysis in U251 cell line lysates (35ug/lane). |
2nd Image Caption |
GGTLC2 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human lung tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. |
3rd Image Caption |
Flow cytometric analysis of U251 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.