Item no. |
PRS-5143-0.1mg |
Manufacturer |
ProSci
|
Amount |
0.1 mg |
Category |
|
Type |
Antibody Polyclonal |
Format |
Liquid |
Applications |
WB, IF, ELISA, IHC-P |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
APP Antibody: AAA, AD1, PN2, ABPP, APPI, CVAP, ABETA, PN-II, CTFgamma, A4, Amyloid beta A4 protein, APP |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Neuroscience |
Homology |
Predicted species reactivity based on immunogen sequence: Pig: (94%), Guinea pig: (94%) |
Immunogen |
APP antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human APP.
The immunogen is located within amino acids 180 - 230 of APP. |
Applications |
APP antibody can be used for detection of APP by Western blot at 1 - 2 μ g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μ g/mL. For immunofluorescence start at 20 μ g/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. |
Positive Control 1 |
Cat. No. 1463 - Rat Brain Tissue Lysate |
Purification |
APP Antibody is affinity chromatography purified via peptide column. |
Clonality |
Polyclonal |
Isotype |
IgG |
Conjugate |
Unconjugated |
Buffer |
APP Antibody is supplied in PBS containing 0.02% sodium azide. |
Concentration |
1 mg/mL |
Storage Conditions |
APP antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Related Products |
Cat. No. 5143P – APP Peptide |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
APP |
Accession # |
CAA30050 |
Protein Gi # |
28721 |
Ncbi Gene Id # |
351 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
APP |
Ncbi Official Full Name |
amyloid beta (A4) precursor protein |
Ncbi Organism |
Homo sapiens |
Swissprot # |
P05067 |
Background |
APP Antibody: Accumulation of the amyloid-beta peptide (Abeta) in the cerebral cortex is a critical event in the pathogenesis of Alzheimer's disease. The beta-amyloid protein precursor (APP) is cleaved by one of two beta-secretases (BACE and BACE2), producing a soluble derivative of the protein and a membrane anchored 99-amino acid carboxy-terminal fragment (C99). The C99 fragment serves as substrate for gamma-secretase to generate the 4 kDa amyloid-beta peptide (Abeta), which is deposited in the Alzheimer's disease patient's brains. Recently, Death Receptor 6 (DR6) was found to interact with an amino-terminal fragment of the Beta-amyloid protein (N-APP) in neurons, activating a caspase 6-dependent apoptotic event leading to axonal degeneration and pruning during development, suggesting that these two proteins are involved in neural development and may possibly play a role in Alzheimer's disease. |
Background References 1 |
Ponte P, Gonzalez-DeWhitt P, Schilling J, et al. A new A4 amyloid mRNA contains a domain homologous to serine proteinase inhibitors. Nature1988; 331:525-77. |
Background References 2 |
Selkoe DJ. Cell biology of the amyloid beta-protein precursor and the mechanism of Alzheimer's disease. Annu Rev Cell Biol1994; 10:373-403. |
Background References 3 |
Nikolaev A, McLaughlin T, O’Leary DD, et al. APP binds DR6 to trigger axon pruning and neuron death via distinct caspases. Nature2009; 457:981-9. |
1st Image Caption |
Western blot analysis of APP in rat brain tissue lysate with APP antibody at (A) 1 and (B) 2 μ g/mL. |
2nd Image Caption |
Immunohistochemistry of APP in mouse brain tissue with APP antibody at 2.5 μ g/mL. |
3rd Image Caption |
Immunofluorescence of APP in mouse brain tissue with APP antibody at 20 μ g/mL. |
4th Image Caption |
Immunohistochemistry of APP in mouse brain tissue with APP Antibody at 5 μ g/mL. |
5th Image Caption |
Immunofluorescence of APP in mouse brain tissue with APP Antibody at 20 μ g/mL.
Green: APP antibody (5143) Red: Phylloidin staining Blue: DAPI staining |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.