Item no. |
PRS-5075-0.02mg |
Manufacturer |
ProSci
|
Amount |
0.02 mg |
Category |
|
Type |
Antibody Polyclonal |
Format |
Liquid |
Applications |
WB, IF, ICC, ELISA |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
OTUD4 Antibody: HIN1, DUBA6, HSHIN1, HIN1, KIAA1046, OTU domain-containing protein 4, HIV-1-induced protein HIN-1 |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Cancer |
Immunogen |
OTUD4 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human OTUD4.
The immunogen is located within the last 50 amino acids of OTUD4. |
Applications |
OTUD4 antibody can be used for detection of OTUD4 by Western blot at 0.25 - 0.5 μ g/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μ g/mL. For immunofluorescence start at 20 μ g/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. |
Specificity |
Multiple isoforms of OTUD4 are known to exist. OTUD4 antibody is predicted to not cross-react with other members of the OTUD family. |
Positive Control 1 |
Cat. No. 1224 - Daudi Cell Lysate |
Predicted Molecular Weight |
Predicted: 115, 123 kDa
Observed: 125 kDa |
Purification |
OTUD4 Antibody is affinity chromatography purified via peptide column. |
Clonality |
Polyclonal |
Isotype |
IgG |
Conjugate |
Unconjugated |
Buffer |
OTUD4 Antibody is supplied in PBS containing 0.02% sodium azide. |
Concentration |
1 mg/mL |
Storage Conditions |
OTUD4 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Related Products |
Cat. No. 5075P – OTUD4 Peptide |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
OTUD4 |
Accession # |
NP_001096123 |
Protein Gi # |
156630992 |
Ncbi Gene Id # |
54726 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
OTUD4 |
Ncbi Official Full Name |
OTU domain containing 4 |
Ncbi Organism |
Homo sapiens |
Swissprot # |
Q01804 |
Background |
OTUD4 Antibody: OTUD4, also known as HIV-1 induced protein HIN-1, is a member of the OTU (ovarian tumor) domain containing cysteine protease superfamily, in which the OUT domain generally confers deubiquitinase activity. At least three isoforms of OTUD4 are known to exist, and the smallest of these isoforms are only expressed in HIV-1-infected cells (provided by RefSeq). |
Background References 1 |
Borodovsky A, Ovaa H, Kolli N, et al. Chemistry-based functional genomics reveals novel members of the deubiquitinating enzyme family. Chem. Biol. 2002; 10:1149-59. |
1st Image Caption |
Western blot analysis of OTUD4 in Daudi cell lysate with OTUD4 antibody at (A) 0.25 and (B) 0.5 μ g/mL. |
2nd Image Caption |
Immunocytochemistry of OTUD4 in Daudi cells with OTUD4 antibody at 2.5 μ g/mL. |
3rd Image Caption |
Immunofluorescence of OTUD4 in Daudi cells with OTUD4 antibody at 20 μ g/mL. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.