Comparison

OTUD4 Antibody

Item no. PRS-5075-0.02mg
Manufacturer ProSci
Amount 0.02 mg
Category
Type Antibody Polyclonal
Format Liquid
Applications WB, IF, ICC, ELISA
Specific against Human, Mouse, Rat
Host Rabbit
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias OTUD4 Antibody: HIN1, DUBA6, HSHIN1, HIN1, KIAA1046, OTU domain-containing protein 4, HIV-1-induced protein HIN-1
Available
Shipping
blue ice or RT
By Research Area
Cancer
Immunogen
OTUD4 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human OTUD4.

The immunogen is located within the last 50 amino acids of OTUD4.
Applications
OTUD4 antibody can be used for detection of OTUD4 by Western blot at 0.25 - 0.5 μ g/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μ g/mL. For immunofluorescence start at 20 μ g/mL.

Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity
Multiple isoforms of OTUD4 are known to exist. OTUD4 antibody is predicted to not cross-react with other members of the OTUD family.
Positive Control 1
Cat. No. 1224 - Daudi Cell Lysate
Predicted Molecular Weight
Predicted: 115, 123 kDa

Observed: 125 kDa
Purification
OTUD4 Antibody is affinity chromatography purified via peptide column.
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Buffer
OTUD4 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration
1 mg/mL
Storage Conditions
OTUD4 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Related Products
Cat. No. 5075P – OTUD4 Peptide
Disclaimer
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications
None
Ncbi Official Symbol
OTUD4
Accession #
NP_001096123
Protein Gi #
156630992
Ncbi Gene Id #
54726
User Note
Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol
OTUD4
Ncbi Official Full Name
OTU domain containing 4
Ncbi Organism
Homo sapiens
Swissprot #
Q01804
Background
OTUD4 Antibody: OTUD4, also known as HIV-1 induced protein HIN-1, is a member of the OTU (ovarian tumor) domain containing cysteine protease superfamily, in which the OUT domain generally confers deubiquitinase activity. At least three isoforms of OTUD4 are known to exist, and the smallest of these isoforms are only expressed in HIV-1-infected cells (provided by RefSeq).
Background References 1
Borodovsky A, Ovaa H, Kolli N, et al. Chemistry-based functional genomics reveals novel members of the deubiquitinating enzyme family. Chem. Biol. 2002; 10:1149-59.
1st Image Caption
Western blot analysis of OTUD4 in Daudi cell lysate with OTUD4 antibody at (A) 0.25 and (B) 0.5 μ g/mL.
2nd Image Caption
Immunocytochemistry of OTUD4 in Daudi cells with OTUD4 antibody at 2.5 μ g/mL.
3rd Image Caption
Immunofluorescence of OTUD4 in Daudi cells with OTUD4 antibody at 20 μ g/mL.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 0.02 mg
Available: In stock
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