Item no. |
PRS-5043-0.1mg |
Manufacturer |
ProSci
|
Amount |
0.1 mg |
Category |
|
Type |
Antibody Polyclonal |
Format |
Liquid |
Applications |
WB, IF, ELISA, IHC-P |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
TMEM18 Antibody: Transmembrane protein 18 |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Stem Cell |
Immunogen |
TMEM18 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human TMEM18.
The immunogen is located within the last 50 amino acids of TMEM18. |
Applications |
TMEM18 antibody can be used for detection of TMEM18 by Western blot at 0.5 - 1 μ g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μ g/mL. For immunofluorescence start at 20 μ g/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. |
Positive Control 1 |
Cat. No. 1463 - Rat Brain Tissue Lysate |
Purification |
TMEM18 Antibody is affinity chromatography purified via peptide column. |
Clonality |
Polyclonal |
Isotype |
IgG |
Conjugate |
Unconjugated |
Buffer |
TMEM18 Antibody is supplied in PBS containing 0.02% sodium azide. |
Concentration |
1 mg/mL |
Storage Conditions |
TMEM18 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Related Products |
Cat. No. 5043P – TMEM18 Peptide |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
TMEM18 |
Accession # |
Q96B42 |
Protein Gi # |
119621502 |
Ncbi Gene Id # |
129787 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
TMEM18 |
Ncbi Official Full Name |
transmembrane protein 18 |
Ncbi Organism |
Homo sapiens |
Swissprot # |
Q96B42 |
Background |
TMEM18 Antibody: Recent studies have examined how overexpression of certain genes enhances the tropism of neural stem cells (NSCs) for gliomas. Overexpression of Transmembrane protein 18 (TMEM18) provides NSCs and neural precursors an increased migration capacity towards glioblastoma cells in vitro and in the rat brain, while functional inactivation of the gene resulted in an almost complete loss of migration activity, suggesting that TMEM18 could be a potential target in NSC-based glioma therapy. Other recent results suggest that among others, the TMEM18 loci is associated with higher than normal body mass index (BMI) and obesity, indicating there may be a CNS-related role in predisposition to obesity. Multiple isoforms of TMEM18 are known to exist. |
Background References 1 |
Jurvansuu J, Zhao Y, Leung DS, et al. Transmembrane protein 18 enhances the tropism of neural stem cells for glioma cells. Cancer Res.2008; 68:4614-22. |
Background References 2 |
Willer CJ, Speliotes EK, Loos RJ, et al. Six new loci associated with body mass index highlight a neuronal influence on body weight regulation. Nat. Genet.2009; 41:25-34. |
Background References 3 |
Renstrom F, Payne F, Nordstrom A, et al. Replication and extension of genome-wide association study results for obesity in 4923 adults from northern Sweden. Human Mol. Gen.2009; 18:1489-96. |
1st Image Caption |
Western blot analysis of TMEM18 in rat brain lysate with TMEM18 antibody at (A) 0.5 and (B) 1 μ g/mL. |
2nd Image Caption |
Immunohistochemistry of TMEM18 in mouse brain tissue with TMEM18 antibody at 2.5 μ g/mL. |
3rd Image Caption |
Immunofluorescence of TMEM18 in Mouse Brain cells with TMEM18 antibody at 20 μ g/mL. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.