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Connexin 43 (phospho Ser368) Antibody

Connexin 43 (phospho Ser368) Antibody

Item no.	PRS-50-220-0.1mL
Manufacturer	ProSci
Amount	0.1 mL
Category	Antibodies Western Blot Primary Antibodies
Type	Antibody Primary
Format	Liquid
Applications	WB
Specific against	Human, Mouse, Rat, Bovine, Canine, Goat, Chicken, Carp
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Available
Shipping	blue ice or RT
By Research Area	Phospho-Specific
Immunogen	Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser368 of rat connexin 43.
Applications	The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with bovine, canine, chicken, guinea pig, human, mouse and zebra fish based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
Predicted Molecular Weight	43
Purification	Affinity Purified
Clonality	Polyclonal
Conjugate	Unconjugated
Storage Conditions	Connexin 43 antibody can be stored at -20˚ C and is stable at -20˚ C for at least 1 year.
Disclaimer	This product is for research use only.
Modifications	Phospho-Specific
Ncbi Official Symbol	Gja1
Accession #	P08050
Protein Gi #	117708
Ncbi Gene Id #	24392
User Note	Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol	Gja1
Ncbi Official Full Name	gap junction protein, alpha 1
Ncbi Organism	Rattus norvegicus
Swissprot #	P08050
Background	Gap junctional intercellular communication is thought to play a key role in development and may also be involved in epilepsy (Aronica et al., 2001). Connexin 43 forms gap-junctional channels and regulates the permeability of these gap junctions to small organic molecules. Permeability of connexin 43 is known to be regulated by phosphorylation at Ser368 by protein kinase C (Yogo et al., 2002; Bao et al., 2004a). Phosphorylation of Ser368 by PKC induces a conformational change of connexin 43 that results in a decrease in gap junction permeability (Bao et al., 2004b).
Background References 1	Aronica E, Gorter JA, Jansen GH, Leenstra S, Yankaya B, Troost D (2001) Expression of connexin 43 and connexin 32 gap-junction proteins in epilepsy-associated brain tumors and in the perilesional epileptic cortex. Acta Neuropathol (Berl) 101:449-459.
Background References 2	Bao X, Altenberg GA, Reuss L (2004a) Mechanism of regulation of the gap junction protein connexin 43 by protein kinase C-mediated phosphorylation. Am J Physiol Cell Physiol 286:C647-C654.
Background References 3	Bao X, Reuss L, Altenberg GA (2004b) Regulation of purified and reconstituted connexin 43 hemichannels by protein kinase C-mediated phosphorylation of Serine 368. J Biol Chem 279:20058-20066.
1st Image Caption	Western blot of rat hippocampal lysate showing specific immunolabeling of the ~43k connexin 43 phosphorylated at Ser368 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lamda-Ptase). The blot is identical to the control except that it was incubated in lamda-Ptase (1200 units for 30 min) before being exposed to the phospho-Ser368 connexin 43 antibody. The immunolabeling of connexin 43 is completely eliminated by treatment with lamda-Ptase.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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