Item no. |
PRS-4995-0.1mg |
Manufacturer |
ProSci
|
Amount |
0.1 mg |
Category |
|
Type |
Antibody Polyclonal |
Format |
Liquid |
Applications |
WB, IF, ELISA, IHC-P |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
IL-16 Antibody: LCF, NIL16, PRIL16, prIL-16, Pro-interleukin-16, Lymphocyte chemoattractant factor, IL-16 |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Immunology |
Immunogen |
IL-16 antibody was raised against a 14 amino acid synthetic peptide near the amino terminus of human IL-16.
The immunogen is located within amino acids 140 - 190 of IL-16. |
Applications |
IL-16 antibody can be used for detection of IL-16 by Western blot at 2.5 - 5 μ g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μ g/mL. For immunofluorescence start at 20 μ g/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. |
Specificity |
This antibody will only detect the NIL-16 isoform. |
Positive Control 1 |
Cat. No. 1463 - Rat Brain Tissue Lysate |
Predicted Molecular Weight |
Predicted: 145 kDa
Observed: 160 kDa |
Purification |
IL-16 Antibody is affinity chromatography purified via peptide column. |
Clonality |
Polyclonal |
Isotype |
IgG |
Conjugate |
Unconjugated |
Buffer |
IL-16 Antibody is supplied in PBS containing 0.02% sodium azide. |
Concentration |
1 mg/mL |
Storage Conditions |
IL-16 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Related Products |
Cat. No. 4995P – IL-16 Peptide |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
IL16 |
Accession # |
AAQ86961 |
Protein Gi # |
36953836 |
Ncbi Gene Id # |
3603 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
IL16 |
Ncbi Official Full Name |
interleukin 16 (lymphocyte chemoattractant factor) |
Ncbi Organism |
Homo sapiens |
Swissprot # |
Q14005 |
Background |
IL-16 Antibody: IL-16 was initially identified as a chemotactic cytokine, but is now known to possess a wide range of activities. Later studies have more fully characterized IL-16 as an immunomodulatory cytokine that contributes to the regulatory process of CD4+ T cell recruitment and activation at sites of inflammation in association with asthma and several autoimmune diseases. The precursor of IL-16 (pro-IL-16) is thought to be cleaved towards the C-terminal region by Caspase-3, releasing a 20 kDa active form that binds to and signals through CD4. Besides acting as a chemotactic cytokine, IL-16 is thought to also be involved in the regulation of T cell proliferation and multiple infectious, immune-mediated, and autoimmune inflammatory disorders including irritable bowel syndrome, systemic lupus erythematosus, and neurodegenerative disorders. At least two isoforms of IL-16 are known to exist; the longer isoform (also known as NIL-16) is detected only in neurons of the cerebellum and hippocampus. |
Background References 1 |
Cruikshank WW, Center DM, Nisar N, et al. Molecular and functional analysis of a lymphocyte chemoattractant factor: association of biologic function with CD expression. Proc. Natl. Acad. Sci. USA1994; 91:5109-13. |
Background References 2 |
Interleukin-16. Cruikshank WW, Kornfeld H, and Center DM. J. Leukoc. Biol.2000; 67:757-66. |
Background References 3 |
Zhang Y, Center DM, Wu DM, et al. Processing and activation of pro-interleukin-16 by caspase-3. J. Biol. Chem.1998; 273:1144-9. |
Background References 4 |
Maciaszek JW, Parada NA, Cruikshank WW, et al. IL-16 represses HIV-1 promoter activity. J. Immunol.1997; 158:5-8. |
1st Image Caption |
Western blot analysis of IL-16 in rat brain tissue lysate with IL-16 antibody at (A) 2.5 and (B) 5 μ g/mL. |
2nd Image Caption |
Immunohistochemistry of IL-16 in mouse brain tissue with IL-16 antibody at 2.5 μ g/mL. |
3rd Image Caption |
Immunofluorescence of IL-16 in mouse brain tissue with IL-16 antibody at 20 μ g/mL. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.